Document Detail

Maternal low-protein diet during mouse pre-implantation development induces vascular dysfunction and altered renin-angiotensin-system homeostasis in the offspring.
MedLine Citation:
PMID:  20128937     Owner:  NLM     Status:  MEDLINE    
Environmental perturbations during early mammalian development can affect aspects of offspring growth and cardiovascular health. We have demonstrated previously that maternal gestational dietary protein restriction in mice significantly elevated adult offspring systolic blood pressure. Therefore, the present study investigates the key mechanisms of blood pressure regulation in these mice. Following mating, female MF-1 mice were assigned to either a normal-protein diet (NPD; 18 % casein) or an isocaloric low-protein diet throughout gestation (LPD; 9 % casein), or fed the LPD exclusively during the pre-implantation period (3.5 d) before returning to the NPD for the remainder of gestation (Emb-LPD). All offspring received standard chow. At 22 weeks, isolated mesenteric arteries from LPD and Emb-LPD males displayed significantly attenuated vasodilatation to isoprenaline (P = 0.04 and P = 0.025, respectively), when compared with NPD arteries. At 28 weeks, stereological analysis of glomerular number in female left kidneys revealed no significant difference between the groups. Real-time RT-PCR analysis of type 1a angiotensin II receptor, Na+/K+ ATPase transporter subunits and glucocorticoid receptor expression in male and female left kidneys revealed no significant differences between the groups. LPD females displayed elevated serum angiotensin-converting enzyme (ACE) activity (P = 0.044), whilst Emb-LPD males had elevated lung ACE activity (P = 0.001), when compared with NPD offspring. These data demonstrate that elevated offspring systolic blood pressure following maternal gestational protein undernutrition is associated with impaired arterial vasodilatation in male offspring, elevated serum and lung ACE activity in female and male offspring, respectively, but kidney glomerular number in females and kidney gene expression in male and female offspring appear unaffected.
Adam J Watkins; Emma S Lucas; Christopher Torrens; Jane K Cleal; Lauren Green; Clive Osmond; Judith J Eckert; William P Gray; Mark A Hanson; Tom P Fleming
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-02-04
Journal Detail:
Title:  The British journal of nutrition     Volume:  103     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-16     Completed Date:  2010-07-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  1762-70     Citation Subset:  IM    
School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, UK.
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MeSH Terms
Blood Pressure*
Bronchodilator Agents / pharmacology
Diet, Protein-Restricted / adverse effects*
Hypertension / etiology*,  physiopathology
Isoproterenol / pharmacology
Kidney / metabolism
Kidney Glomerulus
Lung / metabolism
Maternal Nutritional Physiological Phenomena*
Mice, Inbred Strains
Muscle, Smooth, Vascular / physiopathology
Peptidyl-Dipeptidase A / metabolism*
Pregnancy Complications
Prenatal Exposure Delayed Effects*
Protein Deficiency*
Receptor, Angiotensin, Type 1
Receptors, Glucocorticoid / metabolism
Renin-Angiotensin System / physiology
Reverse Transcriptase Polymerase Chain Reaction
Sex Factors
Sodium-Potassium-Exchanging ATPase
Grant Support
BBF007450//Biotechnology and Biological Sciences Research Council; U01 HD04435/HD/NICHD NIH HHS; //British Heart Foundation
Reg. No./Substance:
0/Bronchodilator Agents; 0/Receptor, Angiotensin, Type 1; 0/Receptors, Glucocorticoid; 7683-59-2/Isoproterenol; EC A; EC ATPase

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