Document Detail

Maternal ghrelin plays an important role in rat fetal development during pregnancy.
MedLine Citation:
PMID:  16339208     Owner:  NLM     Status:  MEDLINE    
Ghrelin, an acylated peptide serving as an endogenous ligand for GH secretagogue receptor (GHS-R), was originally isolated from rat and human stomach. In this study, we report the critical role of maternal ghrelin in fetal development. High levels of ghrelin receptor (GHS-R) mRNA were detected in various peripheral fetal tissues beginning at embryonic d 14 and lasting until birth. Fetal GHS-R expression was also confirmed in fetal tissues by immunohistochemistry. Autoradiography revealed that both des-acyl ghrelin and acyl ghrelin bind to fetal tissues. Chronic treatment of mothers with ghrelin resulted in a significant increase in birth weight in comparison to newborns from saline-treated mothers. Even when maternal food intake after ghrelin treatment was restricted through paired feeding, significant stimulation of fetal development still occurred. Conversely, active immunization of mothers against ghrelin decreased fetal body weight during pregnancy. A single ghrelin injection into the mother increased circulating ghrelin levels in the fetus within 5 min of injection, suggesting that maternal ghrelin transits easily to the fetal circulation. High levels of des-acyl ghrelin were detected in fetal blood and amniotic fluid. Both acylated and des-acyl ghrelin increased [3H]thymidine and 5-bromo-2'-deoxyuridine incorporation of cultured fetal skin cells in a dose-dependent manner, and calcium-imaging analysis revealed that acyl and des-acyl ghrelin increased the Ca2+ influx in discrete cultured fetal skin cells, respectively. These results indicate that maternal ghrelin regulates fetal development during the late stages of pregnancy.
Keiko Nakahara; Mari Nakagawa; Yukiko Baba; Miho Sato; Koji Toshinai; Yukari Date; Masamitsu Nakazato; Masayasu Kojima; Mikiya Miyazato; Hiroyuki Kaiya; Hiroshi Hosoda; Kenji Kangawa; Noboru Murakami
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-08
Journal Detail:
Title:  Endocrinology     Volume:  147     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-16     Completed Date:  2006-04-13     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1333-42     Citation Subset:  AIM; IM    
Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2155, Japan.
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MeSH Terms
Amniotic Fluid / metabolism
Body Weight
Bromodeoxyuridine / pharmacology
Calcium / metabolism
Cell Proliferation
Cells, Cultured
Corticosterone / metabolism
Embryonic Development
Enzyme-Linked Immunosorbent Assay
Gene Expression Regulation, Developmental*
Insulin-Like Growth Factor I / metabolism
Peptide Hormones / blood,  metabolism,  physiology*
Pregnancy, Animal
RNA, Messenger / metabolism
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Reg. No./Substance:
0/Ghrelin; 0/Peptide Hormones; 0/RNA, Messenger; 50-22-6/Corticosterone; 59-14-3/Bromodeoxyuridine; 67763-96-6/Insulin-Like Growth Factor I; 7440-70-2/Calcium

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