Document Detail


Maternal gamma (gamma)-tubulin is involved in microtubule reorganization during bovine fertilization and parthenogenesis.
MedLine Citation:
PMID:  12589656     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, gamma-tubulin distribution was determined chronologically in conjunction with microtubule dynamics during bovine fertilization and parthenogenesis. In unfertilized bovine oocytes, gamma-tubulin was identified in the cytoplasm, mainly in the cortex and concentrated in the meiotic spindle. Following sperm penetration, gamma-tubulin in the cytoplasm was recruited by a sperm component. During pronuclear apposition, gamma-tubulin was localized as spots at the spindle poles. gamma-tubulin spots were observed in blastomeres of embryos cleaved in vitro. Following electrical stimulation, gamma-tubulin and microtubule matrix were noted in oocyte cortex. In the late pronuclear stage, considerably less gamma-tubulin and microtubules were detected in the cytoplasm. At the mitotic metaphase of parthenotes, gamma-tubulin was recruited to the condensed chromatin and concentrated in the spindle. The gamma-tubulin spots were not detected until the 8-cell stage of parthenotes. This suggests that maternal gamma-tubulin is recruited by a sperm component to reconstitute the zygotic centrosome. In the absence of sperm components, the cell cycle-related assembly of gamma-tubulin organizes microtubule nucleation for positioning the pronucleus and spindle protein of mitotic metaphase during the first cell cycle of bovine parthenotes.
Authors:
Mi-Ra Shin; Nam-Hyung Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  64     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-02-17     Completed Date:  2003-10-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  438-45     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheong Ju, Chungbuk, Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Cattle
Centrosome / metabolism
Fertilization / physiology*
Microscopy, Confocal
Microtubules / metabolism*
Parthenogenesis / physiology*
Time Factors
Tubulin / metabolism*
Zygote / metabolism
Chemical
Reg. No./Substance:
0/Tubulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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