| Maternal endotoxin-induced preterm birth in mice: fetal responses in toll-like receptors, collectins, and cytokines. | |
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MedLine Citation:
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PMID: 18287966 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Major cause of prematurity is spontaneous preterm birth (PTB) associated with intrauterine inflammation. Our aim was to establish a model of endotoxin Lipopolysaccharide-induced PTB of live-born pups and to study early immune activation in fetal and maternal compartments. Expression of several proteins that bind microbes (Toll-like receptors TLR4, TLR2; surfactant proteins SP-A, SP-D) was analyzed. At 16 or 17 d of gestation, C57BL/6 dams received a single dose of intraperitoneal LPS, leading to PTB within 17 h. Cytokine levels increased in maternal serum, followed by a modest increase in fetal serum and in amniotic fluid. In uterus, placenta, and fetal membranes, LPS mostly increased the expressions of TLR, SPs, and cytokines. The number of TLR2-positive macrophages increased in labyrinthine placenta. In fetal lung, intestine, liver, and brain there were modest changes in cytokine expressions. In fetal lung, SP and TLR mRNAs decreased and TLR2-positive macrophages redistributed around vessels. LPS-induced fetal deaths associated with early age (16 d gestation) rather than with proinflammatory activation. Here we propose that maternal LPS response leads to PTB and acute decrease of immune proteins in epithelial lining of fetal lung. Instead, acceleration of lung maturity has been previously observed in intraamniotic inflammation. |
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Authors:
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Annamari Salminen; Reija Paananen; Reetta Vuolteenaho; Juhani Metsola; Marja Ojaniemi; Helena Autio-Harmainen; Mikko Hallman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pediatric research Volume: 63 ISSN: 0031-3998 ISO Abbreviation: Pediatr. Res. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-02-21 Completed Date: 2008-03-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0100714 Medline TA: Pediatr Res Country: United States |
Other Details:
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Languages: eng Pagination: 280-6 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, University of Oulu, Oulu, FIN-90014, Finland. annamari.salminen@oulu.fi |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amniotic Fluid
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immunology Animals Chemokine CCL2 / metabolism Collectins / blood, metabolism* Cytokines / blood, metabolism* Extraembryonic Membranes / immunology Female Fetal Blood / immunology Fetal Death Fetus / immunology* Gestational Age Immunohistochemistry Inflammation / chemically induced, immunology*, physiopathology Interleukin-10 / metabolism Interleukin-6 / metabolism Lipopolysaccharides Lung / embryology, immunology Maternal-Fetal Exchange* Mice Mice, Inbred C57BL Placenta / immunology Pregnancy Premature Birth / etiology*, immunology, physiopathology Pulmonary Surfactant-Associated Protein A / metabolism Pulmonary Surfactant-Associated Protein D / metabolism Time Factors Toll-Like Receptor 2 / metabolism Toll-Like Receptor 4 / metabolism Toll-Like Receptors / blood, metabolism* Tumor Necrosis Factor-alpha / metabolism Uterine Diseases / chemically induced, immunology*, physiopathology Uterus / immunology |
| Chemical | |
Reg. No./Substance:
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0/Ccl2 protein, mouse; 0/Chemokine CCL2; 0/Collectins; 0/Cytokines; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Pulmonary Surfactant-Associated Protein A; 0/Pulmonary Surfactant-Associated Protein D; 0/Tlr2 protein, mouse; 0/Tlr4 protein, mouse; 0/Toll-Like Receptor 2; 0/Toll-Like Receptor 4; 0/Toll-Like Receptors; 0/Tumor Necrosis Factor-alpha; 0/lipopolysaccharide, Escherichia coli 0111 B4; 130068-27-8/Interleukin-10 |
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