Document Detail


Maternal clofibrate administration amplifies fetal peroxisomes.
MedLine Citation:
PMID:  2034473     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To study the effects of peroxisome proliferators on fetal biogenesis, 400 mg/kg oral clofibrate was administered to pregnant mice beginning at d 6 of gestation. Maternal/fetal tissues from three separate experiments were harvested between gestational d 13 and 19 and maternal/fetal tissues were assayed for peroxisomal matrix, membrane-associated, and integral membrane proteins. By comparison to control pregnancies that received vehicle only, clofibrate produced a 4- to 5-fold increase in the levels of peroxisomal membrane protein 70, a 1.5- to 2-fold increase of dihydroxyacetone phosphate acyltransferase (DHAP-AT) sp act, and a 1.2- to 1.8-fold increase of catalase sp act in fetal liver of 19 d gestation. Fetal liver endoplasmic reticulum and peroxisomes were also amplified as visualized by electron microscopy. Clofibrate exhibited maximal effects on maternal tissues by 6 d of oral treatment with increases in peroxisomal membrane protein 70 occurring most clearly in maternal liver; DHAP-AT activity was increased in maternal liver, kidney, and brain but not in lung. Slight increases in DHAP-AT activities were evident in fetal brain, lung, and placenta as compared with the greater increases in liver and kidney. There was a general increase in peroxisomal proteins between 13 and 19 gestational d in all fetal tissues except placenta, and the effect of clofibrate was evident by gestational d 13 in lung and placenta. Minimal changes in the activities of acid phosphatase (lysosomal enzyme) or glycerol-3-phosphate acyltransferase (microsomal enzyme) were observed in maternal or fetal tissues although the latter enzyme was increased 10-30% by clofibrate in maternal brain, fetal lung, and placenta.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
G N Wilson; T King; J C Argyle; R F Garcia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pediatric research     Volume:  29     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1991-06-24     Completed Date:  1991-06-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  256-62     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, University of Texas, Southwestern Medical Center, Dallas 75235.
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MeSH Terms
Descriptor/Qualifier:
ATP-Binding Cassette Transporters*
Acyltransferases / metabolism
Animals
Clofibrate / pharmacology*
Female
Fetus / drug effects*,  metabolism,  ultrastructure
Liver / drug effects,  metabolism,  ultrastructure
Maternal-Fetal Exchange
Membrane Proteins / metabolism
Mice
Microbodies / drug effects*,  metabolism,  ultrastructure
Microscopy, Electron
Pregnancy
Chemical
Reg. No./Substance:
0/ATP-Binding Cassette Transporters; 0/Abcd3 protein, mouse; 0/Membrane Proteins; 637-07-0/Clofibrate; EC 2.3.-/Acyltransferases; EC 2.3.1.42/glycerone-phosphate O-acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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