| Maternal cell-free messenger RNA in twin pregnancies: the effects of chorionicity and severe twin to twin transfusion syndrome (TTTS). | |
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MedLine Citation:
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PMID: 22862673 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the effects of chorionicity and severe twin to twin transfusion (TTTS) on maternal circulating cell-free messenger RNA (cf-mRNA). DESIGN: Prospective cohort study. SETTING: A UK tertiary referral Fetal Medicine Center. POPULATION: All monochorionic (MC) twins complicated by severe TTTS (n= 23) and a cohort of uncomplicated dichorionic (DC) (n= 10) and MC (n= 7) pregnancies, between October 2006 and December 2007. METHODS: Maternal cf-mRNA encoding glyceraldehyde 3-phosphate dehydrogenase (GAPDH), vascular endothelial growth factor receptor 1(VEGFR-1(Flt-1)), vascular endothelial growth factor A (VEGF-A), Endoglin, placental growth factor (PlGF), tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie-1), angiopoietin 1 (Ang-1) and angiopoietin 2 (Ang-2) were measured by a quantitative two-step real-time PCR assay after extraction from maternal plasma. RESULTS: The amounts of cf-mRNA detectable are reported for uncomplicated DC, MC and TTTS pregnancies, respectively: GAPDH - 80, 100 and 96%; VEGFR-1 - 10, 0 and 26%; VEGF-A- 80, 71 and 96%; Endoglin-70, 71 and 91%; PlGF-70, 57, 26%; Tie-1 0, 43, 0%; Ang-1 71, 50 and 60% and Ang-2 83, 50 and 89%. There was a significant difference in VEGF-A (medians DC -337.3, MC - 390.8, TTTS - 618.6 copies/mL plasma p= 0.024), Endoglin (medians DC-14.49, MC-1171, TTTS - 2896 copies/mL plasma p= 0.027) and Ang-2 (medians DC-13.66, MC-8.49, TTTS 44.80 copies/mL plasma p= 0.007). CONCLUSION: Maternal cf-mRNA could be reliably detected for GAPDH, PlGF, VEGF-A, Endoglin, Ang-1 and Ang-2 in twin pregnancies and a significant difference was demonstrated in VEGF-A, Endoglin and Ang-2 between uncomplicated twins and MC twin pregnancies complicated by TTTS. If such alterations in maternal cf-mRNA precede the onset of clinically apparent disease, this may be used as an adjuvant blood test to complement ultrasound screening. |
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Authors:
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Caroline E Fox; Akihiko Sekizawa; Samantha J Pretlove; Ben C Chan; Takashi Okai; Mark D Kilby |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-08-21 |
Journal Detail:
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Title: Acta obstetricia et gynecologica Scandinavica Volume: 91 ISSN: 1600-0412 ISO Abbreviation: Acta Obstet Gynecol Scand Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-09-18 Completed Date: 2012-11-19 Revised Date: 2013-02-15 |
Medline Journal Info:
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Nlm Unique ID: 0370343 Medline TA: Acta Obstet Gynecol Scand Country: England |
Other Details:
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Languages: eng Pagination: 1206-11 Citation Subset: IM |
Copyright Information:
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© 2012 The Authors Acta Obstetricia et Gynecologica Scandinavica© 2012 Nordic Federation of Societies of Obstetrics and Gynecology. |
Affiliation:
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Fetal Medicine Centre, Birmingham Women's Foundation Trust, Edgbaston, Birmingham, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Angiopoietin-2 / blood Antigens, CD / blood Biological Markers / blood Case-Control Studies Cell-Free System* Chorion* Cohort Studies Female Fetofetal Transfusion / blood*, diagnosis, genetics Humans Infant, Newborn Pregnancy Prospective Studies RNA, Messenger / blood* Real-Time Polymerase Chain Reaction Receptors, Cell Surface / blood Severity of Illness Index Twins, Monozygotic Vascular Endothelial Growth Factor A / blood |
| Chemical | |
Reg. No./Substance:
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0/Angiopoietin-2; 0/Antigens, CD; 0/Biological Markers; 0/ENG protein, human; 0/RNA, Messenger; 0/Receptors, Cell Surface; 0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A |
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