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Maternal-Restraint Stress Increases Oocyte Aneuploidy by Impairing Metaphase I Spindle Assembly and Reducing Spindle Assembly Checkpoint Proteins in Mice.
MedLine Citation:
PMID:  22133696     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Studies in both humans and animals suggest detrimental effects of psychological stress on reproduction. Although our recent study shows that maternal-restraint stress diminishes oocyte developmental potential, the mechanism is unknown. This promoted us to study the potential role of maternal-restraint stress in the genesis of aneuploidy during meiosis I. At 24 h after eCG injection, mice were subjected to restraint stress for 24 h. After restraint, whereas some mice were killed to recover immature oocytes for in vitro maturation, others were injected with hCG to recover in vivo matured oocytes. Analysis on chromosome complements of both mature oocytes and parthenotes confirmed that maternal restraint increased aneuploidy in both in vivo and in vitro matured oocytes and that percentages of aneuploid oocytes were three times higher in the earlier matured oocytes than in the later matured ones following maternal restraint stress. Further observations indicated that maternal restraint (i) impaired metaphase I spindle assembly while inhibiting MAPK activities; (ii) accelerated progression of anaphase I while down-regulating the expression of spindle checkpoint proteins (SAC); and (iii) induced intra-oocyte oxidative stress. The following possible model was proposed to explain the results. Maternal-restraint stress increased oocyte aneuploidy by impairing MI spindle assembly and decreasing SAC. Whereas abnormal spindles would affect centromere attachments, a reduction in SAC would accelerate the anaphase I progression. Failure of centromere attachment, together with the hastened anaphase, would result in nondisjunction of the unattached chromosomes. Furthermore, maternal restraint stress might have impaired spindle assembly and SAC function by inducing intra-oocyte oxidative stress, which would then reduce the MAPK activity, a critical regulator of microtubule assembly and for the establishment and maintenance of SAC during oocyte maturation.
Authors:
Ping Zhou; Hua-Yu Lian; Wei Cui; De-Li Wei; Qing Li; Yu-Xiang Liu; Xin-Yong Liu; Jing-He Tan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-30
Journal Detail:
Title:  Biology of reproduction     Volume:  -     ISSN:  1529-7268     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-12-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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