Document Detail


Maternal protein restriction reduces expression of angiotensin I-converting enzyme 2 in rat placental labyrinth zone in late pregnancy.
MedLine Citation:
PMID:  22011389     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Both the systemic and the uteroplacental renin-angiotensin system (RAS) display dramatic changes during pregnancy. However, whether gestational protein insufficiency affects the expressions of RAS in the placenta remains unknown. In this study, we hypothesized that the expression of Ace2 in the placental labyrinth was reduced by maternal protein restriction. Pregnant Sprague-Dawley rats were fed a normal diet or a low-protein diet (LP) from Day 1 of pregnancy until they were killed at Day 14 or Day 18. The labyrinth zone (LZ) of the placenta was then dissected and snap frozen for expression analysis by quantitative real-time PCR of Ace, Ace2, Agtr1a, Agtr1b, and Agtr2. Formalin-fixed placentas were used for immunohistochemical analysis on ACE and ACE2 proteins. The findings include 1) the expression of Ace2 in rat LZ was reduced by maternal protein restriction in late pregnancy; 2) ACE protein was mainly present in syncytiotrophoblasts, whereas ACE2 protein was found predominantly in fetal mesenchymal tissue and fetal capillaries; 3) Agtr1a was predominant in the rat LZ, and its mRNA levels, but not protein levels, were reduced by LP; 4) expressions of Ace, Ace2, and Agtr1a in the rat LZ and their response to LP occurred in a gender-dependent manner. These results may indicate that a reduced expression of Ace2 and perhaps an associated reduction in angiotensin (1-7) production in the placenta by maternal protein restriction may be responsible for fetal growth restriction and associated programming of adulthood hypertension.
Authors:
Haijun Gao; Uma Yallampalli; Chandra Yallampalli
Related Documents :
21765069 - Superiority of 3 over 2 doses of intermittent preventive treatment with sulfadoxine-pyr...
22101499 - Stimulation of the endometrium with high-grade blastocyst culture supernatant (sehb) ca...
21843689 - Unintended pregnancy among women in the us military.
21565419 - The impact of the ipledge program on isotretinoin fetal exposure in an integrated healt...
8131399 - Studies on the contraceptive efficacy of praneem polyherbal cream.
21751969 - Impaired endothelial function and elevated levels of pentraxin 3 in early-onset preecla...
10380299 - Screening efficacy of the subcutaneous tissue width/femur length ratio for fetal macros...
11731889 - Perinatal management of fetal cardiac anomalies in a specialized obstetric-pediatrics c...
12699879 - Concentrations of soluble fas in maternal serum and amniotic fluid during uncomplicated...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-02-09
Journal Detail:
Title:  Biology of reproduction     Volume:  86     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-10     Completed Date:  2012-07-09     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  31     Citation Subset:  IM    
Affiliation:
Department of Obstetrics & Gynecology, University of Texas Medical Branch, Galveston, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Angiotensin I / metabolism
Animals
Diet, Protein-Restricted* / adverse effects
Female
Fetal Growth Retardation / chemically induced
Male
Maternal Nutritional Physiological Phenomena / physiology*
Models, Animal
Peptide Fragments / metabolism
Peptidyl-Dipeptidase A / metabolism*
Placenta / metabolism*
Pregnancy
Pregnancy, Animal / metabolism*
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1 / metabolism
Renin-Angiotensin System / physiology
Grant Support
ID/Acronym/Agency:
R01 HL058144/HL/NHLBI NIH HHS; R01 HL102866/HL/NHLBI NIH HHS; R01HL102866/HL/NHLBI NIH HHS; R01HL58144/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Agtr1a protein, rat; 0/Peptide Fragments; 0/RNA, Messenger; 0/Receptor, Angiotensin, Type 1; 0/angiotensin I (1-7); 9041-90-1/Angiotensin I; EC 3.4.15.1/Peptidyl-Dipeptidase A; EC 3.4.17.-/angiotensin converting enzyme 2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comparative effectiveness of nafcillin or cefazolin versus vancomycin in methicillin-susceptible Sta...
Next Document:  Spata22, a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells.