Document Detail

Maternal protein restriction reduces expression of angiotensin I-converting enzyme 2 in rat placental labyrinth zone in late pregnancy.
MedLine Citation:
PMID:  22011389     Owner:  NLM     Status:  MEDLINE    
Both the systemic and the uteroplacental renin-angiotensin system (RAS) display dramatic changes during pregnancy. However, whether gestational protein insufficiency affects the expressions of RAS in the placenta remains unknown. In this study, we hypothesized that the expression of Ace2 in the placental labyrinth was reduced by maternal protein restriction. Pregnant Sprague-Dawley rats were fed a normal diet or a low-protein diet (LP) from Day 1 of pregnancy until they were killed at Day 14 or Day 18. The labyrinth zone (LZ) of the placenta was then dissected and snap frozen for expression analysis by quantitative real-time PCR of Ace, Ace2, Agtr1a, Agtr1b, and Agtr2. Formalin-fixed placentas were used for immunohistochemical analysis on ACE and ACE2 proteins. The findings include 1) the expression of Ace2 in rat LZ was reduced by maternal protein restriction in late pregnancy; 2) ACE protein was mainly present in syncytiotrophoblasts, whereas ACE2 protein was found predominantly in fetal mesenchymal tissue and fetal capillaries; 3) Agtr1a was predominant in the rat LZ, and its mRNA levels, but not protein levels, were reduced by LP; 4) expressions of Ace, Ace2, and Agtr1a in the rat LZ and their response to LP occurred in a gender-dependent manner. These results may indicate that a reduced expression of Ace2 and perhaps an associated reduction in angiotensin (1-7) production in the placenta by maternal protein restriction may be responsible for fetal growth restriction and associated programming of adulthood hypertension.
Haijun Gao; Uma Yallampalli; Chandra Yallampalli
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-02-09
Journal Detail:
Title:  Biology of reproduction     Volume:  86     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-10     Completed Date:  2012-07-09     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  31     Citation Subset:  IM    
Department of Obstetrics & Gynecology, University of Texas Medical Branch, Galveston, USA.
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MeSH Terms
Angiotensin I / metabolism
Diet, Protein-Restricted* / adverse effects
Fetal Growth Retardation / chemically induced
Maternal Nutritional Physiological Phenomena / physiology*
Models, Animal
Peptide Fragments / metabolism
Peptidyl-Dipeptidase A / metabolism*
Placenta / metabolism*
Pregnancy, Animal / metabolism*
RNA, Messenger / metabolism
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1 / metabolism
Renin-Angiotensin System / physiology
Grant Support
Reg. No./Substance:
0/Agtr1a protein, rat; 0/Peptide Fragments; 0/RNA, Messenger; 0/Receptor, Angiotensin, Type 1; 0/angiotensin I (1-7); 9041-90-1/Angiotensin I; EC A; EC 3.4.17.-/angiotensin converting enzyme 2

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