Document Detail


Maternal Low-protein Diet Alters Ovarian Expression of Folliculogenic and Steroidogenic Genes and Their Regulatory MicroRNAs in Neonatal Piglets.
MedLine Citation:
PMID:  25358362     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Maternal malnutrition during pregnancy may give rise to female offspring with disrupted ovary functions in adult age. Neonatal ovary development predisposes adult ovary function, yet the effect of maternal nutrition on the neonatal ovary has not been described. Therefore, here we show the impact of maternal protein restriction on the expression of folliculogenic and steroidogenic genes, their regulatory microRNAs and promoter DNA methylation in the ovary of neonatal piglets. Sows were fed either standard-protein (SP, 15% crude protein) or low-protein (LP, 7.5% crude protein) diets throughout gestation. Female piglets born to LP sows showed significantly decreased ovary weight relative to body weight (p<0.05) at birth, which was accompanied with an increased serum estradiol level (p<0.05). The LP piglets demonstrated higher ratio of bcl-2 associated X protein/B cell lymphoma/leukemia-2 mRNA (p<0.01), which was associated with up-regulated mRNA expression of bone morphogenic protein 4 (BMP4) (p<0.05) and proliferating cell nuclear antigen (PCNA) (p<0.05). The steroidogenic gene, cytochrome P450 aromatase (CYP19A1) was significantly down-regulated (p<0.05) in LP piglets. The alterations in ovarian gene expression were associated with a significant down-regulation of follicle-stimulating hormone receptor mRNA expression (p<0.05) in LP piglets. Moreover, three microRNAs, including miR-423-5p targeting both CYP19A1 and PCNA, miR-378 targeting CYP19A1 and miR-210 targeting BMP4, were significantly down-regulated (p<0.05) in the ovary of LP piglets. These results suggest that microRNAs are involved in mediating the effect of maternal protein restriction on ovarian function through regulating the expression of folliculogenic and steroidogenic genes in newborn piglets.
Authors:
Shiyan Sui; Yimin Jia; Bin He; Runsheng Li; Xian Li; Demin Cai; Haogang Song; Rongkui Zhang; Ruqian Zhao
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian-Australasian journal of animal sciences     Volume:  27     ISSN:  1011-2367     ISO Abbreviation:  Asian-australas. J. Anim. Sci.     Publication Date:  2014 Dec 
Date Detail:
Created Date:  2014-10-31     Completed Date:  2014-10-31     Revised Date:  2014-11-05    
Medline Journal Info:
Nlm Unique ID:  9884245     Medline TA:  Asian-Australas J Anim Sci     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  1695-704     Citation Subset:  -    
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