Document Detail

Maternal administration of erythromycin fails to eradicate intrauterine ureaplasma infection in an ovine model.
MedLine Citation:
PMID:  20610808     Owner:  NLM     Status:  MEDLINE    
Erythromycin is the standard antibiotic used for treatment of infection with Ureaplasma spp. during pregnancy; however, maternally administered erythromycin may be ineffective at eliminating intra-amniotic ureaplasma infections. We examined whether erythromycin would eradicate intra-amniotic ureaplasma infections in pregnant sheep. At Gestational Day (GD) 50 (term, GD 150), pregnant ewes received intra-amniotic injections of erythromycin-sensitive Ureaplasma parvum serovar 3 (n = 16) or 10B medium (n = 16). At GD 100, amniocentesis was performed; five fetal losses (ureaplasma group, n = 4; 10B group, n = 1) had occurred by this time. Remaining ewes were allocated into treatment subgroups: medium only (n = 7), medium and erythromycin (n = 8), ureaplasma only (Up; n = 6), or ureaplasma and erythromycin (Up/E; n = 6). Erythromycin was administered intramuscularly (500 mg) every 8 h for 4 days (GDs 100-104). Amniotic fluid samples were collected at GD 105. At GD 125, preterm fetuses were surgically delivered, and specimens were collected for culture and histology. Erythromycin was quantified in amniotic fluid by liquid chromatography-mass spectrometry. Ureaplasmas were isolated from the amniotic fluid, chorioamnion, and fetal lung of animals from the Up and Up/E groups, however, the numbers of U. parvum recovered were not different between these groups. Inflammation in the chorioamnion, cord, and fetal lung was increased in ureaplasma-exposed animals compared to controls but was not different between the Up and Up/E groups. Erythromycin was detected in amniotic fluid samples, although concentrations were low (<10-76 ng/ml). This study demonstrates that maternally administered erythromycin does not eradicate chronic, intra-amniotic ureaplasma infections or improve fetal outcomes in an ovine model, potentially because of the poor placental passage of erythromycin.
Samantha J Dando; Ilias Nitsos; John P Newnham; Alan H Jobe; Timothy J M Moss; Christine L Knox
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-07
Journal Detail:
Title:  Biology of reproduction     Volume:  83     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-22     Completed Date:  2011-01-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  616-22     Citation Subset:  IM    
Institute of Health & Biomedical Innovation, Faculty of Science and Technology, Queensland University of Technology, Brisbane, Queensland, Australia.
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MeSH Terms
Amniotic Fluid / chemistry,  microbiology
Anti-Bacterial Agents / administration & dosage*,  pharmacokinetics
Colony Count, Microbial / veterinary
DNA, Bacterial / chemistry,  genetics
Erythromycin / administration & dosage*,  pharmacokinetics
Extraembryonic Membranes / chemistry,  microbiology
Histocytochemistry / veterinary
Injections, Intramuscular / veterinary
Lung Diseases / drug therapy,  embryology,  microbiology,  veterinary*
Polymerase Chain Reaction / veterinary
Pregnancy Complications, Infectious / drug therapy,  microbiology,  veterinary*
Sheep Diseases / drug therapy,  embryology*,  microbiology
Ureaplasma / genetics,  growth & development*
Ureaplasma Infections / drug therapy,  embryology,  microbiology,  veterinary*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/DNA, Bacterial; 114-07-8/Erythromycin

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