Document Detail

Material-driven differentiation of induced pluripotent stem cells in neuron growth factor-grafted poly(ε-caprolactone)-poly(β-hydroxybutyrate) scaffolds.
MedLine Citation:
PMID:  22591608     Owner:  NLM     Status:  Publisher    
The potential of constructs comprising induced pluripotent stem (iPS) cells and biopolymers can be high for neurological surgery practice, if the systematic activity of neuronal regeneration is clarified. This study shows a guided differentiation of iPS cells toward neurons in neuron growth factor (NGF)-grafted poly(ε-caprolactone) (PCL)-poly(β-hydroxybutyrate) (PHB) scaffolds. The porosity of PCL-PHB scaffolds enhanced with increasing the concentration of salt particles (porogen) and the weight percentage of PCL. An increase in the graft concentration of NGF elevated the atomic ratios of N/C and O/C on the surface of NGF-grafted PCL-PHB scaffolds. In addition, incorporating heparin and NGF promoted the adhesion and viability of iPS cells in constructs. When the weight percentage of PCL increased, the viability of iPS cells reduced; however, more PCL in constructs benefited the adhesion of iPS cells. Under the influence of heparin and NGF, a high weight percentage of PCL and a long inductive period improved iPS cells to differentiate into neuron-like cells carrying βIII tubulin and inhibited other differentiation(s). The material-driven differentiation in NGF-grafted PCL-PHB constructs can be promising in guiding iPS cells to produce neurons for nerve tissue engineering.
Yung-Chih Kuo; Min-Jung Huang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-14
Journal Detail:
Title:  Biomaterials     Volume:  -     ISSN:  1878-5905     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Department of Chemical Engineering, National Chung Cheng University, Chia-Yi 62102, Taiwan, Republic of China.
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