| Matched work high-intensity interval and continuous running induce similar increases in PGC-1α mRNA, AMPK, p38 and p53 phosphorylation in human skeletal muscle. | |
| | |
MedLine Citation:
|
PMID: 22267390 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The aim of the present study was to test the hypothesis that acute high-intensity interval (HIT) running induces greater activation of signalling pathways associated with mitochondrial biogenesis compared with moderate-intensity continuous (CONT) running matched for work done. In a repeated measures design, ten active men performed two running protocols consisting of HIT (6 x 3-min at 90% VO(2max) interspersed with 3-min recovery periods at 50% VO(2max) with a 7-min warm up and cool down period at 70% VO(2max)) or CONT (50-min continuous running at 70% VO(2max)). Both protocols were matched, therefore, for average intensity, duration and distance ran. Muscle biopsies (vastus lateralis) were obtained pre-exercise, post-exercise and 3 h post-exercise. Muscle glycogen decreased (P < 0.05) similarly in HIT and CONT (116 ± 11 v 111 ± 17 mmol.kg(-1) dw, respectively). Phosphorylation (P-) of p38MAPK(Thr180/Tyr182) (1.9 ± 0.1 v 1.5 ± 0.2-fold) and AMPK(Thr172) (1.5 ± 0.3 v 1.5 ± 0.1-fold) increased immediately post-exercise (P < 0.05) in HIT and CONT, respectively, and returned to basal levels at 3 h post-exercise. P-p53(Ser15) (HIT: 2.7 ± 0.8-fold; CONT: 2.1 ± 0.8-fold), PGC-1α mRNA (HIT:4.2 ± 1.7-fold: CONT: v 4.5 ± 0.9-fold) and HSP72 mRNA (HIT: 4.4 ± 2-fold; CONT: 3.5 ± 1-fold) all increased 3 h post-exercise (P < 0.05) though neither parameter increased (P > 0.05) immediately post-exercise. There was no difference between trials for any of the above signalling or gene expression responses (P > 0.05). We provide novel data by demonstrating that acute HIT and CONT running (when matched for average intensity, duration and work done) induces similar activation of molecular signalling pathways associated with regulation of mitochondrial biogenesis. Furthermore, this is the first report of contraction-induced p53 phosphorylation in human skeletal muscle, thus highlighting an additional pathway by which exercise may initiate mitochondrial biogenesis. |
| | |
Authors:
|
Jonathan D Bartlett; Chang Hwa-Joo; Tae-Seok Jeong; Jari Louhelainen; Andrew J Cochran; Martin J Gibala; Warren Gregson; Graeme L Close; Barry Drust; James P Morton |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-19 |
Journal Detail:
|
Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
1Liverpool John Moores University. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Dyspnea-pain counter-irritation induced by inspiratory threshold loading: a laser evoked potentials ...
Next Document: Regular and moderate exercise before experimental sepsis reduces the risk of lung and distal organ i...