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Match criteria for human cell line authentication: Where do we draw the line?
MedLine Citation:
PMID:  23136038     Owner:  NLM     Status:  Publisher    
Continuous human cell lines have been used extensively as models for biomedical research. In working with these cell lines, researchers are often unaware of the risk of cross-contamination and other causes of misidentification. To reduce this risk, there is a pressing need to authenticate cell lines, comparing the sample handled in the laboratory to a previously tested sample. The American Type Culture Collection Standards Development Organization Workgroup ASN-0002 has developed a Standard for human cell line authentication, recommending short tandem repeat (STR) profiling for authentication of human cell lines. However, there are known limitations to the technique when applied to cultured samples, including possible genetic drift with passage. In the current study, a dataset of 2279 STR profiles from four cell banks was used to assess the effectiveness of the match criteria recommended within the Standard. Of these 2279 STR profiles, 1157 were grouped into sets of related cell lines - duplicate holdings, legitimately related samples, or misidentified cell lines. Eight core STR loci plus amelogenin were used to unequivocally authenticate 98 % of these related sets. Two simple match algorithms each clearly discriminated between related and unrelated samples, with separation between related samples at ≥80 % match and unrelated samples at <50 % match. A small degree of overlap was noted at 50 % to 79 % match, mostly from cell lines known to display variable STR profiles. These match criteria are recommended as a simple and effective way to interpret results from STR profiling of human cell lines. © 2012 Wiley Periodicals, Inc.
Amanda Capes-Davis; Yvonne A Reid; Margaret C Kline; Douglas R Storts; Ethan Strauss; Wilhelm G Dirks; Hans G Drexler; Roderick A F Macleod; Gregory Sykes; Arihiro Kohara; Yukio Nakamura; Eugene Elmore; Raymond W Nims; Christine Alston-Roberts; Rita Barallon; Georgyi V Los; Roland M Nardone; Paul J Price; Anton Steuer; Jim Thomson; John R W Masters; Liz Kerrigan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  -     ISSN:  1097-0215     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 UICC.
CellBank Australia - Children's Medical Research Institute, Westmead, New South Wales, Australia.
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