Document Detail


Mast cells prevent dexamethasone-induced cell death of cultured fibroblasts: relationship to gap junctional intercellular communications.
MedLine Citation:
PMID:  24776565     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Dexamethasone, a common therapy for reducing hypertrophic scar, sometimes fails. However, in cell culture, all dexamethasone-treated fibroblasts die. In co-cultures, gap junction intercellular communications between mast cells and fibroblasts promote profibrotic activities. Does the co-culture of mast cells with fibroblasts prevent dexamethasone-induced fibroblast death?
METHODS: Survival of fibroblasts co-cultured with RMC-1 cells, a rat mast cell line, receiving dexamethasone was studied. RMC-1 cells pretreated with a secretagogue that degranulated mast cells and/or with a long-acting gap junction intercellular communications inhibitor were compared to untreated RMC-1 cells co-cultured with fibroblasts and dexamethasone.
RESULTS: Fibroblasts alone treated with dexamethasone all died in 3 hours. Fibroblasts co-cultured with intact RMC-1 cells or with degranulated RMC-1 cells in dexamethasone all survived. No fibroblasts survived, co-cultured with RMC-1 cells unable to form gap junction intercellular communications with fibroblasts.
CONCLUSIONS: Dexamethasone-treated fibroblasts, forming gap junction intercellular communications with mast cells, may explain why dexamethasone therapy sometimes fails. Gap junction intercellular communications between scar mast cells and fibroblasts or myofibroblasts apparently blocks the death of these cell populations. Preventing gap junction intercellular communications between mast cells and fibroblasts by including anti-gap junction intercellular communication agents may enhance the effectiveness of steroid therapy in treating excessive scarring.
Authors:
Theodore T Foley; H Paul Ehrlich
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plastic and reconstructive surgery     Volume:  133     ISSN:  1529-4242     ISO Abbreviation:  Plast. Reconstr. Surg.     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-04-29     Completed Date:  2014-06-26     Revised Date:  2014-10-13    
Medline Journal Info:
Nlm Unique ID:  1306050     Medline TA:  Plast Reconstr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  638e-644e     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*,  physiology
Cell Communication / drug effects*,  physiology
Cell Line
Cicatrix, Hypertrophic / drug therapy,  pathology
Coculture Techniques
Cytoplasmic Granules / drug effects,  physiology
Dexamethasone / pharmacology*
Fibroblasts / cytology*,  drug effects
Foreskin / cytology
Gap Junctions / drug effects*,  physiology
Glucocorticoids / pharmacology
Humans
Keloid / drug therapy,  pathology
Male
Mast Cells / cytology*
Rats
Chemical
Reg. No./Substance:
0/Glucocorticoids; 7S5I7G3JQL/Dexamethasone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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