Document Detail

Mast cells in the seminal plasma of infertile men as detected by flow cytometry.
MedLine Citation:
PMID:  19143722     Owner:  NLM     Status:  MEDLINE    
Increased numbers of mast cells (MCs) in the testis have been associated with testicular dysfunction, where accumulation of MCs occurs. Furthermore, it has been reported that MCs might affect sperm function as it has been demonstrated that MC-derived tryptase in the seminal fluid might reduce sperm motility. Although MCs have been detected in rat epididymis, only little is known about the presence of MCs in human seminal plasma. Thus, we analysed MC numbers in the ejaculate of men during routine semen analysis of male patients suspected for infertility (n = 100). MCs were detected by c-kit (CD117) expression using flow cytometry. Thereby, we detected significant numbers of MCs in the ejaculate of most patients (559 +/- 525 MCs ml(-1), mean +/- SD). However, we could neither detect a correlation with respect to MCs and sperm count, motility or morphology nor to the seminal inflammatory markers like polymorphonuclear elastase. Nevertheless, a significant correlation of MCs to spermatozoa-bound IgA (r = 0.5; P = 0.03; n = 21) was observed. It is concluded that significant numbers of MCs can be detected in the human ejaculate without necessarily influencing sperm function. A potential role of MCs in seminal plasma as well as the association between MCs and IgA on spermatozoa remains to be elucidated.
J-P Allam; M Langer; A Fathy; I Oltermann; T Bieber; N Novak; G Haidl
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Andrologia     Volume:  41     ISSN:  1439-0272     ISO Abbreviation:  Andrologia     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-15     Completed Date:  2009-03-20     Revised Date:  2011-12-20    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1-6     Citation Subset:  IM    
Andrology Unit, Department of Dermatology and Allergy, University Hospital Bonn, Bonn, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Flow Cytometry
Immunoglobulin A / metabolism
Infertility, Male / pathology*
Leukocyte Elastase / analysis
Mast Cells / pathology*
Neutrophils / enzymology
Semen / cytology*
Semen Analysis
Sperm Count
Sperm Motility
Spermatozoa / immunology,  metabolism
Reg. No./Substance:
0/Immunoglobulin A; EC Elastase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The TEG vs the ROTEM thromboelastography/thromboelastometry systems.
Next Document:  Concurrent improvement of the metabolic syndrome and lower urinary tract symptoms upon normalisation...