| Mast cells and eosinophils in the allergic mucosal response to allergen challenge: changes in distribution and signs of activation in relation to symptoms. | |
| | |
MedLine Citation:
|
PMID: 1281177 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
An allergen challenge was performed in 10 asymptomatic patients with strictly seasonal allergic rhinitis. For comparison; seven nonallergic subjects were challenged with allergen, and seven allergic patients were challenged with diluent. Cell samples, obtained with use of a brush technique to recover cells from within the epithelium and nasal lavage to collect cells from the epithelial surface, and symptom scores were taken before challenge and at 2-hour intervals during 12 hours. The cell suspensions were cytocentrifuged onto object slides for light microscopy. Histamine was determined in the cell pellets. In brush samples from the allergic patients challenged with allergen, eosinophils, expressed as a percentage of the total granulocytes, increased from 4.3% +/- 2.7% (mean +/- SEM) to 10.3% +/- 3.8% (p < 0.05) 4 hours after challenge. This level was maintained for up to 12 hours. A similar increase was noted in the lavage specimens 2, 6, and 8 hours after the challenge. In the brush samples the proportion of eosinophils containing two or more cytoplasmic vacuoles, taken as a sign of activation, increased from 20% to 72% (p < 0.05) 8 hours after provocation. In brush samples from the allergic patients challenged with allergen, the numbers of metachromatic cells increased to a maximum of eightfold at 10 hours. In the lavage specimens, no metachromatic cells were observed before provocation, but they progressively increased in number 2 to 12 hours after provocation. Cell pellet histamine content decreased temporarily 2 to 4 hours after challenge (p < 0.05) in brush samples from allergen-challenged allergic patients. The local metachromatic cell density before challenge, as reflected in the brush specimens, correlated with nasal congestion, sneezing, and the degree of eosinophilia. |
| | |
Authors:
|
S Juliusson; U Pipkorn; G Karlsson; L Enerbäck |
Related Documents
:
|
9758817 - Determination of the biomasses of small bacteria at low concentrations in a mixture of ... 3118567 - Application of quantifiable criteria in the lukes and collins classification of non-hod... 11865407 - Rhinovirus infection increases 5-lipoxygenase and cyclooxygenase-2 in bronchial biopsy ... 18228357 - Isolation of peroxisomes from tissues and cells by differential and density gradient ce... 14684377 - Sdf-1/cxcl12 regulates camp production and ion transport in intestinal epithelial cells... 21056967 - Regulation of m₃ muscarinic receptor expression and function by transmembrane protein... |
Publication Detail:
|
Type: Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of allergy and clinical immunology Volume: 90 ISSN: 0091-6749 ISO Abbreviation: J. Allergy Clin. Immunol. Publication Date: 1992 Dec |
Date Detail:
|
Created Date: 1993-01-12 Completed Date: 1993-01-12 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 1275002 Medline TA: J Allergy Clin Immunol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 898-909 Citation Subset: AIM; IM |
Affiliation:
|
Department of Otorhinolaryngology, Sahlgrenska Hospital, University of Göteborg, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Allergens / administration & dosage* Cell Count Eosinophils / cytology* Female Histamine / analysis Humans Leukocyte Count Male Mast Cells / cytology* Nasal Mucosa / chemistry Nasal Provocation Tests Neutrophils / cytology Rhinitis, Allergic, Perennial / pathology* Staining and Labeling Time Factors Tolonium Chloride |
| Chemical | |
Reg. No./Substance:
|
0/Allergens; 51-45-6/Histamine; 92-31-9/Tolonium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Butyrylcholinesterase-rich neurons in rat brain demonstrated by a sensitive histochemical method.
Next Document: Identification of common allergenic structures in hazel pollen and hazelnuts: a possible explanation...