| Mast cells associate with neovessels in the media and adventitia of abdominal aortic aneurysms. | |
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MedLine Citation:
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PMID: 19515525 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Mast cells (MCs) are inflammatory cells present in atherosclerotic lesions and neovascularized tissues. Recently, MCs were shown to modulate abdominal aortic aneurysm (AAA) formation in a mouse model. Progression of aneurysmatic disease process may also depend on intraluminal thrombus and neovascularization of the aneurysm wall. Here we investigated the relationship between MCs and inflammation, neovascularization, and the presence of intraluminal thrombus in human AAA. METHODS AND RESULTS: Specimens from AAAs and normal control aortas were analyzed with basic histology, immunohistochemical staining, and quantitative real-time polymerase chain reaction (PCR). Double immunostainings with endothelial cell markers CD31/CD34 and MC tryptase showed that, in contrast to histologically normal aorta, MCs in AAA were abundant in the media, but absent from the intima. Medial MCs and (CD31/CD34)(+) neovessels increased significantly in AAA compared with normal aorta (P < .0001 for both), and the highest densities of neovessels and MCs were observed in the media of thrombus-covered AAA samples. Also, the proportional thickness of aortic wall penetrated by the neovessels was significantly higher in the AAA samples (P < .0001), and the neovascularized area correlated with the density of medial MCs (P < .0001). In histologic analysis, the medial MCs were mainly located adjacent to the stem cell factor (SCF)(+) medial neovessels. Real-time PCR analysis also showed that mRNA levels of genes associated with neovascularization (vascular endothelial growth factor [VEGF], FLT1, VE-cadherin, CD31), and MCs (tryptase, chymase, cathepsin G) were higher in AAA samples than in controls. Demonstration of adhered platelets by CD42b staining and lack of endothelial cell (CD31/CD34) staining in the luminal surface of AAA specimens suggest endothelial erosion of the aneurysm walls. CONCLUSIONS: The results support participation of MCs in the pathogenesis of AAA, particularly regarding neovascularization of aortic wall. |
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Authors:
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Mikko I Mäyränpää; Julia A Trosien; Vincent Fontaine; Maggie Folkesson; Monsur Kazi; Per Eriksson; Jesper Swedenborg; Ulf Hedin |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-10 |
Journal Detail:
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Title: Journal of vascular surgery : official publication, the Society for Vascular Surgery [and] International Society for Cardiovascular Surgery, North American Chapter Volume: 50 ISSN: 1097-6809 ISO Abbreviation: J. Vasc. Surg. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-27 Completed Date: 2009-09-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8407742 Medline TA: J Vasc Surg Country: United States |
Other Details:
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Languages: eng Pagination: 388-95; discussion 395-6 Citation Subset: IM |
Affiliation:
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Wihuri Research Institute, Helsinki, Finland. mikko.mayranpaa@helsinki.fi |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Aortic Aneurysm, Abdominal / metabolism, pathology* Biological Markers / metabolism Case-Control Studies Connective Tissue / metabolism, pathology* Disease Progression Female Humans Immunohistochemistry Inflammation / metabolism, pathology Male Mast Cells / pathology* Neovascularization, Pathologic / metabolism, pathology* Photomicrography Platelet Aggregation Reverse Transcriptase Polymerase Chain Reaction Statistics, Nonparametric Thrombosis / metabolism, pathology Tryptases / metabolism Tunica Intima / metabolism, pathology Tunica Media / metabolism, pathology Vascular Endothelial Growth Factor A / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Vascular Endothelial Growth Factor A; EC 3.4.21.59/Tryptases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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