| Mast cells and MMP-9 in the lamina propria during eruption of rat molars: quantitative and immunohistochemical evaluation. | |
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MedLine Citation:
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PMID: 20557403 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During the active tooth eruption process, structural changes in the lamina propria are necessary to provide extracellular matrix remodelling and for the establishment of the eruptive pathway. A large number of resident cells, recruited cells and proteases have been demonstrated in the eruptive process, but the participation of MMP-9 and mast cells has not yet been demonstrated. In this study, we set out to evaluate the intensity of MMP-9 immunoexpression, the frequency of mast cells and the correlation between the incidence of mast cells and bone resorption in different phases of tooth eruption. Fragments of maxilla containing first molars, obtained from 9-, 11-, 13- and 16-day-old rats, were fixed in 4% formaldehyde, decalcified and embedded in paraffin. Sagittal sections were stained with Masson's trichrome or submitted to the tartrate-resistant acid phosphatase method for quantification of osteoclasts. Sections stained by 1% toluidine blue were used for quantification of metachromatic mast cells mm(-2) of lamina propria. The expression of MMP-9 in the lamina propria was evaluated by immunohistochemistry. In the 9-day-old rats, the lamina propria contained few mast cells and occasional osteoclasts were found in the bone surface overlying the occlusal portion of the tooth germs. Otherwise, a significant increase in the number of mast cells was observed in the intra-osseous phase of tooth eruption (11-day-old rats), period in which numerous TRAP-positive osteoclasts were found in the bone surface. MMP-9 immunolabelling was detected in fibroblasts, mast cells and macrophage-like cells of the lamina propria in all ages studied. However, an enhanced immunolabelling was evident in the advanced phase of tooth eruption (16-day-old rats). During the intra-osseous phase, the parallel between the high frequency of both mast cells and osteoclasts suggests that mast cells could exert a paracrine function on the osteoclasts and then stimulate bone resorption. The immunoexpression of MMP-9 in different cells of lamina propria, including mast cells, indicates that this enzyme participates in the degradation of ECM, mainly during late phase of mucosal penetration. Thus mast cells and MMP-9 are involved in the complex process of degradation of the eruptive pathway extracellular matrix. |
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Authors:
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Paulo Sérgio Cerri; Jorge Alonso Pereira-Júnior; Natalia Barrionuevo Biselli; Estela Sasso-Cerri |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-16 |
Journal Detail:
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Title: Journal of anatomy Volume: 217 ISSN: 1469-7580 ISO Abbreviation: J. Anat. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-16 Completed Date: 2011-01-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0137162 Medline TA: J Anat Country: England |
Other Details:
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Languages: eng Pagination: 116-25 Citation Subset: IM |
Affiliation:
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Department of Morphology, Laboratory of Histology and Embryology, Dental School, UNESP - Univ. Estadual Paulista, Araraquara, São Paulo, Brazil. pcerri@foar.unesp.br |
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| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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metabolism Animals Biological Markers / metabolism Cell Count Immunoenzyme Techniques Isoenzymes / metabolism Male Mast Cells / enzymology* Matrix Metalloproteinase 9 / metabolism* Molar / cytology*, enzymology Mucous Membrane / cytology*, enzymology Osteoclasts / cytology, enzymology Rats Tooth Eruption / physiology* |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Isoenzymes; EC 3.1.3.-/tartrate-resistant acid phosphatase; EC 3.1.3.2/Acid Phosphatase; EC 3.4.24.35/Matrix Metalloproteinase 9 |
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