| Mast cell typing: demonstration of a distinct hematopoietic cell type and evidence for immunophenotypic relationship to mononuclear phagocytes. | |
| | |
MedLine Citation:
|
PMID: 2469499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The immunologic surface marker profile of human mast cells (MCs) was established using a combined toluidine/immunofluorescence staining procedure [49 monoclonal antibodies (MoAbs) tested]. Ascites (n = 9) MCs as well as enzymatically dispersed MCs from all organs tested (lung n = 11, skin n = 7, intestinum n = 10) exhibited an identical marker profile. MCs were recognized by MoAbs clustered as CD9 (anti-gp24), CD33 (anti-gp67), and CD45 (anti-gp220) as well as by MoAbs directed against membrane-bound IgE. MoAB YB5B8 (anti-gp145) selectively recognized MCs. Most significantly, however, MCs were stained by MoAbs MAX1 (anti-gp65), MAX3 (anti-gp68), MAX11 (anti-gp65), and MAX24 (anti-gp65). These antibodies bind to surface membrane antigens associated with a late stage of monocyte/macrophage differentiation. Thus, our results provide definite evidence that MCs share surface membrane markers with mononuclear phagocytes. In contrast, MCs are stained neither by lymphatic markers (CD1-8, 10, 19-24) nor by myelomonocytic markers (CD11-17). MCs also lack the interleukin-2 (IL-2) receptor (CD25), the T10 antigen (CD38), and most of the myelocytic markers expressed on peripheral blood (PB) basophils. Thus, MCs displayed a unique phenotype within the hematopoietic system. This new approach enabled us to enrich human lung MCs to a purity greater than 95% by means of negative selection with complement-mediated cell lysis. Purified MCs were subsequently stained with MoAbs and analyzed by flow cytometry, which confirmed the results obtained from the double-staining experiments. We next examined cultured metachromatic cells derived from bone marrow (BM) and peripheral blood colony-forming units (CFU). These metachromatic cells previously could not be classified by morphologic criteria alone and have therefore been termed basophil-like/MC-like cells. In this study, toluidine blue-positive cells obtained from either pooled multipotential colonies (day 14-CFU-GEM) or pooled myelocytic colonies (day 16/17-CFU-GM/G/M) were recognized by MoAbs MY7 (CD13), VIM12 (CD11b), and VIM2, as well as by an anti-IgE MoAb, after preincubation with IgE. In contrast, CFU-derived metachromatic cells were not stained by MoAb YB5B8. This marker profile corresponds to the immunologic phenotype of blood basophils and excluded a detectable formation of mature MCs in colonies derived from cultured hematopoietic stem cells. |
| | |
Authors:
|
P Valent; L K Ashman; W Hinterberger; F Eckersberger; O Majdic; K Lechner; P Bettelheim |
Related Documents
:
|
9688999 - Vascular and glomerular expression of endothelin-1 in normal human kidney. 10559639 - Antisense and kidney cell research. 16260769 - Endosymbiotic yeast maternally transmitted in a marine sponge. 15539379 - Relative contributions of enzyme cytochemistry and flow cytometric immunophenotyping to... 15735749 - Mesenchymal cells generated from patients with myelodysplastic syndromes are devoid of ... 3495689 - Leukocyte analysis using monoclonal antibodies in human glomerulonephritis. 9315659 - Hepatocyte nuclear factor 3beta is involved in pancreatic beta-cell-specific transcript... 7820049 - Investigating hairy cell leukemia dysregulations. looking for interferon alpha site of ... 4013909 - The non-mast cell histamine in the submandibular gland of the cat. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Blood Volume: 73 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 1989 May |
Date Detail:
|
Created Date: 1989-06-16 Completed Date: 1989-06-16 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7603509 Medline TA: Blood Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1778-85 Citation Subset: AIM; IM |
Affiliation:
|
I. Medical Department, University of Vienna, Austria. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antigens, Surface
/
analysis* Basophils / classification, metabolism Cell Count Cell Separation Child Child, Preschool Flow Cytometry Hematopoietic Stem Cells / classification*, metabolism Histamine Release Humans Lung Mast Cells / classification*, metabolism Neuraminidase Phagocytes / classification*, metabolism Phenotype Staining and Labeling |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, Surface; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interleukin-3 is a differentiation factor for human basophils.
Next Document: Characterization of human marrow stromal cells: role in progenitor cell binding and granulopoiesis.