| Mast cell density and substance P-like immunoreactivity during the initiation and progression of lung lesions in ovine Mannheimia (Pasteurella) haemolytica pneumonia. | |
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MedLine Citation:
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PMID: 11399139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine the density of mast cells (MCs) and the extent of substance P (SP) immunoreactivity during initiation and progression of pneumonic pasteurellosis (PP), 18 lambs were inoculated intrabronchially with Mannheimia (Pasteurella) haemolytica or saline, and lung tissue was collected at 1, 15 and 45 days post-inoculation (n=3, each group). Additionally, the left (non-inoculated) contralateral lungs in bacteria-inoculated animals were collected as controls. At 1 day after bacterial inoculation the lungs had typical M. haemolytica lesions. These pneumonic lesions had fewer numbers of MCs and reduced histamine content. Macrophages infiltrating some of the inflamed areas were strongly immunoreactive for SP. At 15 days, MCs remained scarce at sites where lung damage persisted, i.e. pyogranulomatous foci, but were increased in number in areas of interstitial damage. Pulmonary ganglion neurons were strongly immunoreactive for SP. By 45 days the fibrosing changes became more defined as pleural fibrosis, fibrosing alveolitis, alveolar epithelial hyperplasia and bronchiolitis obliterans. These lungs had increased numbers of MCs, but histamine content was not different from saline- and non-inoculated left lungs. Substance P immunoreactivity occurred only in nerves and was scarce and mild. This work demonstrates that MC density decreases initially with PP, but increases with progression of PP. SP fibres tend to be decreased during the initiation and at 45 days of PP, but other cells, such as macrophages and neuronal ganglion cells, produce substance P during progression of PP and thereby constitute an additional source of substance P. |
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Authors:
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R Ramírez-Romero; K A Brogden; J M Gallup; I M Sonea; M R Ackermann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Microbial pathogenesis Volume: 30 ISSN: 0882-4010 ISO Abbreviation: Microb. Pathog. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-06-11 Completed Date: 2001-08-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8606191 Medline TA: Microb Pathog Country: England |
Other Details:
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Languages: eng Pagination: 325-35 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Academic Press. |
Affiliation:
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Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Female Histamine / analysis Immunohistochemistry Lung / pathology* Male Mannheimia haemolytica* Mast Cells / pathology* Pasteurellosis, Pneumonic / etiology, immunology, pathology* Sheep Sheep Diseases / etiology, immunology, pathology* Substance P / isolation & purification* |
| Chemical | |
Reg. No./Substance:
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33507-63-0/Substance P; 51-45-6/Histamine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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