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Mass spectrometry-based metabolomic profiling identifies alterations in salivary redox status and fatty acid metabolism in response to inflammation and oxidative stress in periodontal disease.
MedLine Citation:
PMID:  24607715     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Periodontal diseases represent the most common chronic inflammatory diseases in humans and a major cause of tooth loss. Combining mass spectrometry-based ionomics and a targeted lipidomics on fatty acids metabolites, we identified significant alterations in redox status and fatty acids metabolism in saliva in response to chronic inflammation and oxidative stress in periodontal disease in a cohort of nonsmoker subjects with chronic periodontitis. For the first time, ionomic profiling of around 30 ions in saliva revealed a significantly decreased levels of redox active metal ions including Mn, Cu, and Zn in periodontal group, which is consistent with a decreased levels of superoxide dismutases (SODs) in saliva and serum. A targeted lipidomic approach was employed to monitor the major metabolites of arachidonic acid and linoleic acid in saliva. We observed increased levels of cyclooxygenase (COX) products including PGE2, PGD2, and PGF2α, TXB2, while decreased level of PGI2 in periodontal group. A unique pattern of the lipoxygenase (LOX) products of arachidonic acid and linoleic acid was observed with increased level of 5-HETE but decreased levels of 13-HODE and 9-HODEs. Levels of salivary F2-isoprostanes, free radical lipid peroxidation products and a gold standard for oxidative stress in vivo, are also significantly elevated. Taken these data together, our study using multiple powerful omics techniques demonstrated that local redox alteration contributes significantly to periodontitis through the modulation of fatty acid metabolisms in response to inflammation and oxidative stress. This study highlights the importance of redox status in periodontitis and provides rationale to prevent periodontal disease by dietary interventions aiming to restore redox balance.
Authors:
Yijing Huang; Mingjiang Zhu; Zi Li; Rina Sa; Qianqian Chu; Qingli Zhang; Haifeng Zhang; Wen Tang; Meifang Zhang; Huiyong Yin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-3-6
Journal Detail:
Title:  Free radical biology & medicine     Volume:  -     ISSN:  1873-4596     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-3-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Elsevier Inc. All rights reserved.
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