Document Detail

Mass spectrometry based N- and C-terminal sequence determination of a hepatopancreas-type prophenoloxidase from the kuruma prawn, Marsupenaeus japonicus.
MedLine Citation:
PMID:  23283287     Owner:  NLM     Status:  Publisher    
We previously identified and characterized a novel hepatopancreas-type prophenoloxidase from kuruma prawn, Marsupenaeus japonicus. In the characterization, this enzyme was indicated to have a feature of a signal peptide at its N-terminus. The putative primary structure was then proposed but its N- and C-terminal sequences remained undetermined. In the present study, the N- and C-terminal amino acid sequences of this prophenoloxidase were determined by de novo sequencing methods using matrix-assisted laser desorption ionization mass spectrometry. The sequence analyses revealed that the N-terminus of the prophenoloxidase was processed, whereas the C-terminus was not. This finding suggests that this enzyme has a signal peptide, and that it is synthesized at the endoplasmic reticulum in hepatopancreas cells and secreted to hemolymph plasma, similar to the case of hemocyanin, another member of the class III copper proteins.
Hiroki Kuyama; Taro Masuda; Chihiro Nakajima; Kyosuke Momoji; Tatsuya Sugawara; Osamu Nishimura; Takashi Hirata
Related Documents :
2447867 - Seminal plasma peptides inhibiting rna synthesis.
23486227 - Production of disulfide-stabilized transmembrane peptide complexes for structural studies.
24801507 - Rnadigest: a web-based tool for the analysis and prediction of structure - specific rna...
23209237 - Draft genome sequence of streptomyces sp. strain ss, which produces a series of uridyl ...
11552677 - Structure-function analysis of d9n and n291s mutations in human lipoprotein lipase usin...
15927697 - Identification and molecular cloning of a novel neuromedin u analog from the skin secre...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-3
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  -     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Division of Disease Proteomics, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka, 565-0871, Japan,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Graphene-modified electrode. Determination of hydrogen peroxide at high concentrations.
Next Document:  Liquid crystalline inorganic nanosheets for facile synthesis of polymer hydrogels with anisotropies ...