Document Detail

Mass spectrometry-based strategies for screening of bioactive natural products.
MedLine Citation:
PMID:  21118084     Owner:  NLM     Status:  In-Data-Review    
Natural products (NPs) are combinatorial chemical libraries with diversities in chemical structures and pharmacological activities. Screening active compounds is in many cases an important factor in drug discovery. It was not easy to screen out the bioactive compounds from complex extracts consisting of many NPs. Development of rapid, effective and accurate methods is in high demand. During last decades, mass spectrometry (MS)-based strategies, combining isolation, structures, and bioactivity in a single run, were programmed in the NPs screening. The current article reviews different assay formats and applications of MS-based methods for screening of active NPs. This review is divided into three sections based on methods classification. The first part introduces binding-based screening methods that directly assess the binding characteristics of a candidate molecule to its target. The second part describes function-based screening methods that monitor the functional output of a target-dependent biochemical reaction. The third part briefly discusses serum pharmacochemistry-based screening methods that analyze absorbed components and metabolites in plasma after oral administration of NPs extracts.
Gui-Zhong Xin; Jian-Liang Zhou; Lian-Wen Qi; Ping Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Combinatorial chemistry & high throughput screening     Volume:  14     ISSN:  1875-5402     ISO Abbreviation:  Comb. Chem. High Throughput Screen.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810948     Medline TA:  Comb Chem High Throughput Screen     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  93-103     Citation Subset:  IM    
Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education and Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China.
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