| Mass Spectrometric Distinction of In-Source and In-Solution Pyroglutamate and Succinimide in Proteins: A Case Study on rhG-CSF. | |
| | |
MedLine Citation:
|
PMID: 23283728 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Formation of cyclic intermediates involving water or ammonia loss is a common occurrence in any reaction involving terminal amines or hydroxyl group containing species. Proteins that have both these functional groups in abundance are no exception, and presence of amino acids such as asparagine, glutamines, aspartic acids, and glutamic acids aid in formation of such intermediates. In the biopharma scenario, such intermediates lead to product- or process-related impurities that might be immunogenic. Mass spectroscopy is a powerful technique that is used to decipher the presence and physicochemical characteristics of such impurities. However, such intermediates can also form in situ during mass spectrometric analysis. We present here the detection of in-source and in-solution formation of succinimide and pyroglutamate in the protein granulocyte colony stimulating factor. We also propose an approach for quick differentiation of such in-situ species from the tangible impurities. We believe that this will not only reduce the time spent in unambiguous identification of succinimide- and/or pyroglutamate-related impurity in bio-pharmaceutics but also provide a platform for similar studies on other impurities that may form due to stabilized intermediates. |
| | |
Authors:
|
Mukesh Kumar; Amarnath Chatterjee; Anand P Khedkar; Mutyalasetty Kusumanchi; Laxmi Adhikary |
Related Documents
:
|
3007268 - Lack of glycosilation of pro-opiomelanocortin might account for the periodic acid-schif... 1964878 - Purification and characterization of a nuclease (3'-nucleotidase) from a penicillium sp. 3861608 - Biosynthesis of polymyxin e by a cell-free enzyme system. iv. acylation of enzyme-bound... 10970788 - The skin of atopic dermatitis patients contains a novel enzyme, glucosylceramide sphing... 7253848 - Regulation of 3-hydroxy-3-methylglutaryl coa reductase by analogs of cholesterol and bi... 8350088 - A novel amino acid glycoside and three amino acids from allium sativum. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-3 |
Journal Detail:
|
Title: Journal of the American Society for Mass Spectrometry Volume: - ISSN: 1879-1123 ISO Abbreviation: J. Am. Soc. Mass Spectrom. Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9010412 Medline TA: J Am Soc Mass Spectrom Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Molecular Characterization Laboratory, Biocon Research Ltd., Bangalore, 560099, Karnataka, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comprehensive Analysis of LC/MS Data Using Pseudocolor Plots.
Next Document: An Improved Measurement of Isotopic Ratios by High Resolution Mass Spectrometry.