Document Detail


Mass spectrometric analysis of sialylated glycans with use of solid-phase labeling of sialic acids.
MedLine Citation:
PMID:  23445396     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The analysis of sialylated glycans is critical for understanding the role of sialic acid in normal biological processes as well as in disease. However, the labile nature of sialic acid typically renders routine analysis of this monosaccharide by mass spectrometric methods difficult. To overcome this difficulty we pursued derivatization methodologies, extending established acetohydrazide approaches to aniline-based methods, and finally to optimized p-toluidine derivatization. This new quantitative glycoform profiling method with use of MALDI-TOF in positive ion mode was validated by first comparing N-glycans isolated from fetuin and serum and was then exploited to analyze the effects of increased metabolic flux through the sialic acid pathway in SW1990 pancreatic cancer cells by using a colabeling strategy with light and heavy toluidine. The latter results established that metabolic flux, in a complementary manner to the more well-known impact of sialyltransferase expression, can critically modulate the sialylation of specific glycans while leaving others virtually unchanged.
Authors:
Punit Shah; Shuang Yang; Shisheng Sun; Paul Aiyetan; Kevin J Yarema; Hui Zhang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-03-18
Journal Detail:
Title:  Analytical chemistry     Volume:  85     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-02     Completed Date:  2013-11-18     Revised Date:  2014-04-04    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3606-13     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Line, Tumor
Fetuins / chemistry
Humans
Polysaccharides / chemistry*
Serum / chemistry
Sialic Acids / analysis*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Grant Support
ID/Acronym/Agency:
N01−HV-00240/HV/NHLBI NIH HHS; P01 HL107153/HL/NHLBI NIH HHS; P01HL107153/HL/NHLBI NIH HHS; R01 CA112314/CA/NCI NIH HHS; R01CA112314/CA/NCI NIH HHS; U01 CA152813/CA/NCI NIH HHS; U01CA152813/CA/NCI NIH HHS; U24CA160036/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Fetuins; 0/Polysaccharides; 0/Sialic Acids
Comments/Corrections

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