Document Detail

Maslinic acid, a natural triterpenoid compound from Olea europaea, protects cortical neurons against oxygen-glucose deprivation-induced injury.
MedLine Citation:
PMID:  21839077     Owner:  NLM     Status:  Publisher    
Maslinic acid is a triterpenoid compound present in plants of Olea europaea. This compound has been reported to have potent antioxidant, anti-cancer, anti-HIV and anti-inflammatory activities. In this study, we investigated the neuroprotective effect of maslinic acid and its mechanism of action. With presence or absence of maslinic acid, cortical neurons were subjected to 1h of oxygen-glucose deprivation and 24h of reoxygenation. Cell injury was determined by lactate dehydrogenase (LDH) measurement and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. Neuronal apoptosis was evaluated by flow cytometry assay, caspase-3 expression/activity, caspase-9 activity and Bcl-2/Bax ratio. Nitric Oxide (NO) production and inducible nitric oxide synthase (iNOS) expression were also detected. Results showed that maslinic acid dose-dependently ameliorated neuron injury and apoptosis. Maslinic acid treatment normalized the caspase expression/activation and increased the Bcl-2/Bax ratio. In addition, maslinic acid inhibited oxygen-glucose deprivation-induced NO production and iNOS expression. These results indicated that maslinic acid has beneficial effects on hypoxic neurons by suppressing iNOS activation, which may, in turn, provide neuroprotection.
Yisong Qian; Teng Guan; Xuzhen Tang; Longfei Huang; Menghao Huang; Yunman Li; Hongbin Sun
Related Documents :
8053927 - Impact of dietary folic acid on reduced folates in mouse plasma and tissues. relationsh...
14099997 - The assay of folic-acid activity of liver.
3513567 - Oral folic acid versus placebo in the treatment of males with the fragile x syndrome.
16197587 - Effect of folic acid supplementation on mood and serotonin response in healthy males.
3858227 - The effect of sweeteners on acid production in plaque.
9083677 - Spontaneous transfer of monoacyl amphiphiles between lipid and protein surfaces.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-5
Journal Detail:
Title:  European journal of pharmacology     Volume:  -     ISSN:  1879-0712     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier B.V.
Department of Physiology, China Pharmaceutical University, 24 Tongjiaxiang Street, Nanjing 210009, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Early changes induced by environmental stresses in insulin sensitivity-related genes.
Next Document:  Protective role of l-carnitine supplementation against exhaustive exercise-induced oxidative stress ...