| Masking of epitopes in tissue sections. A study of glial fibrillary acidic (GFA) protein with antisera and monoclonal antibodies. | |
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MedLine Citation:
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PMID: 6084655 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Antisera to chicken brain antigen (CBA) isolated by hydroxyapatite chromatography from 8 M urea extracts following repeated extractions with phosphate buffer selectively decorate neurofilaments (NF) in neuronal perikarya, dendrites and axons. The antisera also reacted with GFA protein, the astrocyte-specific intermediate filament protein, as indicated by the adsorption of NF immunoreactivity following passage of the antisera through columns prepared with purified GFA protein. Moreover, the antisera stained the polypeptides of the NF triplet (70 kd, 150 kd, 200 kd) and GFA protein by the immunoblotting procedure. Monoclonal antibodies selectively decorating NF in tissue sections were isolated from a fusion of mouse myeloma cells with spleen cells of mice immunized with CBA. By the immunoblotting procedure the antibodies decorated the 150 kd NF polypeptide and GFA protein. No staining of glial filaments or any other structure on tissue sections was also observed with antibodies derived from another fusion strongly reacting with GFA protein on immunoblots. All antibodies (monoclonal and polyclonal) appeared to react with the same region of the GFA polypeptide as indicated by immunoblots of cleavage products. |
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Authors:
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D Dahl; M Grossi; A Bignami |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Histochemistry Volume: 81 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1984 |
Date Detail:
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Created Date: 1985-04-17 Completed Date: 1985-04-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 525-31 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal Antigen-Antibody Complex Cattle Chickens Epitopes / analysis* Glial Fibrillary Acidic Protein / analysis*, immunology Immune Sera Optic Nerve / analysis Purkinje Cells / cytology Spinal Cord / analysis, cytology |
| Grant Support | |
ID/Acronym/Agency:
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NS 13034/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigen-Antibody Complex; 0/Epitopes; 0/Glial Fibrillary Acidic Protein; 0/Immune Sera |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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