Document Detail


Masking of epitopes in tissue sections. A study of glial fibrillary acidic (GFA) protein with antisera and monoclonal antibodies.
MedLine Citation:
PMID:  6084655     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antisera to chicken brain antigen (CBA) isolated by hydroxyapatite chromatography from 8 M urea extracts following repeated extractions with phosphate buffer selectively decorate neurofilaments (NF) in neuronal perikarya, dendrites and axons. The antisera also reacted with GFA protein, the astrocyte-specific intermediate filament protein, as indicated by the adsorption of NF immunoreactivity following passage of the antisera through columns prepared with purified GFA protein. Moreover, the antisera stained the polypeptides of the NF triplet (70 kd, 150 kd, 200 kd) and GFA protein by the immunoblotting procedure. Monoclonal antibodies selectively decorating NF in tissue sections were isolated from a fusion of mouse myeloma cells with spleen cells of mice immunized with CBA. By the immunoblotting procedure the antibodies decorated the 150 kd NF polypeptide and GFA protein. No staining of glial filaments or any other structure on tissue sections was also observed with antibodies derived from another fusion strongly reacting with GFA protein on immunoblots. All antibodies (monoclonal and polyclonal) appeared to react with the same region of the GFA polypeptide as indicated by immunoblots of cleavage products.
Authors:
D Dahl; M Grossi; A Bignami
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Histochemistry     Volume:  81     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1984  
Date Detail:
Created Date:  1985-04-17     Completed Date:  1985-04-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  525-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Antigen-Antibody Complex
Cattle
Chickens
Epitopes / analysis*
Glial Fibrillary Acidic Protein / analysis*,  immunology
Immune Sera
Optic Nerve / analysis
Purkinje Cells / cytology
Spinal Cord / analysis,  cytology
Grant Support
ID/Acronym/Agency:
NS 13034/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigen-Antibody Complex; 0/Epitopes; 0/Glial Fibrillary Acidic Protein; 0/Immune Sera

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