Document Detail


Marsupial light chains: IGK with four V families in the opossum Monodelphis domestica.
MedLine Citation:
PMID:  10630297     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A full-length and several partial cDNAs encoding IGK light chains from the marsupial South American opossum, Monodelphis domestica, were isolated and characterized. Using these clones as a starting point, the expressed IGKV repertoire was sampled by anchored polymerase chain reaction using an IGKC-specific primer. Based on nucleotide sequences of twenty unique, expressed IGKV-J combinations, there are at least four IGKV families and two J segments. Southern blot analysis revealed each IGK-V family contains multiple gene segments totaling at least thirty-five IGKV in the opossum genome. No evidence for particular, recurrent IGKV-J combinations in the opossum IGK repertoire was seen, rather the V-J combinations appeared random and diverse. Each of the four IGKV families appear more closely related to V segments from placental mammals than to each other, suggesting the duplication of the IGKV families prior to the separation of marsupials and placental mammals more than one-hundred-million years ago. Overall, the complexity of opossum light chain V segments appears greater than that found in the heavy chain, and light chains are likely to contribute significantly to Ig diversity in this species. With this report, the homologues encoding all three classes of eutherian Ig chains, IGH, IGL, and IGK, have been described in a non-placental mammal.
Authors:
R D Miller; E R Bergemann; G H Rosenberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Immunogenetics     Volume:  50     ISSN:  0093-7711     ISO Abbreviation:  Immunogenetics     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-01-27     Completed Date:  2000-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0420404     Medline TA:  Immunogenetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  329-35     Citation Subset:  IM    
Affiliation:
Department of Biology, University of New Mexico, Albuquerque 87131, USA. rdmiller@unm.edu
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF116921;  AF116922;  AF116923;  AF116924;  AF116925;  AF116926;  AF116927;  AF116928;  AF116929;  AF116930;  AF116931;  AF116932;  AF116933;  AF116934;  AF116935;  AF116936;  AF116937;  AF116938;  AF116939;  AF116940;  AF116941;  AF116942
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Blotting, Southern
Gene Library
Genes, Immunoglobulin / genetics*
Humans
Immunoglobulins / genetics*
Molecular Sequence Data
Opossums / genetics*,  immunology
Phylogeny
Sequence Homology, Amino Acid
Chemical
Reg. No./Substance:
0/IgK; 0/Immunoglobulins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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