| Marsupial cardiac myosins are similar to those of eutherians in subunit composition and in the correlation of their expression with body size. | |
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MedLine Citation:
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PMID: 16988832 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cardiac myosins and their subunit compositions were studied in ten species of marsupial mammals. Using native gel electrophoresis, ventricular myosin in macropodoids showed three isoforms, V(1), V(2) and V(3), and western blots using specific anti-alpha- and anti-beta-cardiac myosin heavy chain (MyHC) antibodies showed their MyHC compositions to be alphaalpha, alphabeta and betabeta, respectively. Atrial myosin showed alphaalpha MyHC composition but differed from V(1) in light chain composition. Small marsupials (Sminthopsis crassicaudata, Antechinus stuartii, Antechinus flavipes) showed virtually pure V(1), while the larger (1-3 kg) Pseudocheirus peregrinus and Trichosurus vulpecula showed virtually pure V(3). The five macropodoids (Bettongia penicillata, Macropus eugenii, Wallabia bicolour, M. rufus and M. giganteus), ranging in body mass from 2 to 66 kg, expressed considerably more alpha-MyHC (22.8%) than expected for their body size. These results show that cardiac myosins in marsupial mammals are substantially the same as their eutherian counterparts in subunit composition and in the correlation of their expression with body size, the latter feature underlies the scaling of resting heart rate and cardiac cross-bridge kinetics with specific metabolic rate. The data from macropodoids further suggest that expression of cardiac myosins in mammals may also be influenced by their metabolic scope. |
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Authors:
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Joseph F Y Hoh; Yoonah Kim; Jacqueline H Y Lim; Louise G Sieber; Christine A Lucas; Wendy W H Zhong |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-09-20 |
Journal Detail:
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Title: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology Volume: 177 ISSN: 0174-1578 ISO Abbreviation: J. Comp. Physiol. B, Biochem. Syst. Environ. Physiol. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-01-31 Completed Date: 2007-09-27 Revised Date: 2009-06-08 |
Medline Journal Info:
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Nlm Unique ID: 8413200 Medline TA: J Comp Physiol B Country: Germany |
Other Details:
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Languages: eng Pagination: 153-63 Citation Subset: IM |
Affiliation:
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Discipline of Physiology and the Bosch Institute, Building F13, School of Medical Sciences, Faculty of Medicine, The University of Sydney, Sydney, NSW, 2006, Australia. joeh@physiol.usyd.edu.au |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Body Size / physiology* Cardiac Myosins / analysis, chemistry*, physiology Electrophoresis, Polyacrylamide Gel Immunohistochemistry Macropodidae / physiology* Marsupialia / physiology* Myocardium / chemistry, cytology Protein Isoforms |
| Chemical | |
Reg. No./Substance:
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0/Protein Isoforms; EC 3.6.1.-/Cardiac Myosins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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