Document Detail


Marmoset liver cytochrome P450s: study for expression and molecular cloning of their cDNAs.
MedLine Citation:
PMID:  9056237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Northern blot and immunoblot analyses indicated that considerable levels of CYP2B, CYP2C, CYP2D, CYP2E, and CYP3A were expressed in the liver of untreated marmosets. CYP1A was also expressed but to lesser extents. CYP3A mRNA was also detectable in the small intestine of untreated marmoset; the amount was increased by treatment with polychlorinated biphenyl. From a liver cDNA library, two cDNA clones coding for CYP2D19 and CYP3A21 (clones CM2D-1 and CM3A-10, respectively) were isolated. CM2D-1 and CM3A-10 contained an entire coding region for polypeptide 497 and 503 amino acid residues, respectively. The deduced amino acid sequences of CYP2D19 and CYP3A21 showed 90% identities to human CYP2D6 and CYP3A4, respectively. The value of CYP3A21 was 3% lower than that of cynomolgus monkey CYP3A8. On the other hand, these values were 11 to 23% higher than those of the other experimental animals, including dogs, rabbits, guinea pigs, rats, mice, and hamsters. These results indicate that the marmoset stands at a midpoint between human and nonprimate experimental animals.
Authors:
T Igarashi; T Sakuma; M Isogai; R Nagata; T Kamataki
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  339     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-04-16     Completed Date:  1997-04-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  85-91     Citation Subset:  IM    
Affiliation:
Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/D29822;  D31921
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Aryl Hydrocarbon Hydroxylases*
Base Sequence
Blotting, Western
Callithrix / metabolism*
Cattle
Cloning, Molecular
Cricetinae
Cytochrome P-450 Enzyme System / genetics*,  metabolism*
DNA, Complementary / genetics
Dogs
Female
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Guinea Pigs
Humans
Mice
Microsomes, Liver / enzymology*
Molecular Sequence Data
Molecular Weight
Rabbits
Rats
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
0/DNA, Complementary; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/cytochrome P-450 CYP2D19 (marmoset); EC 1.14.14.1/cytochrome P-450 CYP3A21 (marmoset)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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