Document Detail


Markers to evaluate the quality and self-renewing potential of engineered human skin substitutes in vitro and after transplantation.
MedLine Citation:
PMID:  18719609     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We screened a series of antibodies for their exclusive binding to the human hair follicle bulge. In a second step these antibodies were to be used to identify basal keratinocytes and potential epithelial stem cells in the human epidermis and in engineered skin substitutes. Of all the antibodies screened, we identified only one, designated C8/144B, that exclusively recognized the hair follicle bulge. However, C8/144B-binding cells were never detected in the human epidermal stratum basale. In the bulge C8/144B-binding cells gave rise to cytokeratin 19-positive cells, which were also tracked in the outer root sheath between bulge and the hair follicle matrix. Remarkably, cytokeratin 19-expressing cells were never detected in the hair follicle infundibulum. Yet, cytokeratin 19-expressing keratinocytes were found in the epidermal stratum basale of normal skin as a subpopulation of cytokeratin 15-positive (not C8/144B-positive) basal keratinocytes. Cytokeratin 19/cytokeratin 15-positive keratinocytes decreased significantly with age. We suggest that cytokeratin 19-expressing cells represent a subpopulation of basal keratinocytes in neonates and young children (up to 1.5 years) that is particularly adapted to the lateral expansion of growing skin. Our data show that cytokeratin 19 in combination with cytokeratin 15 is an important marker to routinely monitor epidermal homeostasis and (at least indirectly) the self-renewing potential of engineered skin.
Authors:
Luca Pontiggia; Thomas Biedermann; Martin Meuli; Daniel Widmer; Sophie Böttcher-Haberzeth; Clemens Schiestl; Jörg Schneider; Erik Braziulis; Irene Montaño; Claudia Meuli-Simmen; Ernst Reichmann
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-21
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  129     ISSN:  1523-1747     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-16     Completed Date:  2009-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  480-90     Citation Subset:  IM    
Affiliation:
Tissue Biology Research Unit, Department of Surgery, University Children's Hospital, Zurich, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Antibody Specificity
Biological Markers / metabolism*
Cells, Cultured
Epidermis / cytology
Fibroblasts / cytology,  immunology,  metabolism
Hair Follicle / cytology,  immunology,  metabolism
Humans
Immunohistochemistry
Keratin-15 / immunology,  metabolism
Keratin-19 / immunology,  metabolism
Keratinocytes / cytology,  immunology,  metabolism
Keratins, Hair-Specific / immunology,  metabolism
Organ Culture Techniques
Quality Control
Skin Transplantation / methods*,  standards
Skin, Artificial / standards*
Tissue Engineering*
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Keratin-15; 0/Keratin-19; 0/Keratins, Hair-Specific

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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