| Markers of mitochondrial biogenesis and metabolism are lower in overweight and obese insulin-resistant subjects. | |
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MedLine Citation:
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PMID: 17244782 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Impaired mitochondrial function in skeletal muscle is implicated in the development of insulin resistance. However, potential differences in fatness and fitness may influence previous results. METHODS: Subjects (n=18) were divided into insulin-sensitive (IS) and insulin-resistant (IR) groups by median glucose infusion rate during a hyperinsulinemic euglycemic clamp. Weight, VO2max (maximal aerobic capacity), and percentage body fat were measured before and after 6 continuous weeks of aerobic exercise training at 55-70% VO2max (40 min/session, 4 d/wk). RESULTS: Age, percentage fat, and VO2max were not different between IS and IR groups at baseline. Expression of the nuclear encoded PGC1alpha and mitochondrial encoded gene COX1 were significantly lower in the IR group (P<0.05). Citrate synthase activity and protein levels of subunits from complexes I and III of the respiratory chain were also lower in the IR group (P<0.05). Insulin sensitivity and aerobic fitness were increased after exercise training in both groups (P<0.001), and the expression of mitochondrial encoded genes CYTB and COX1 was also increased (P<0.01). However, there was no change in PGC1alpha expression, mitochondrial enzyme activity, or protein levels of complexes of the respiratory chain in response to exercise in either group. CONCLUSION: This study confirms that IR men have reduced markers of mitochondrial metabolism, independent of fatness and fitness. Moderate exercise training did not alter these markers despite improving fitness and whole body insulin sensitivity. This study suggests that additional mechanisms may be involved in improving insulin resistance after exercise training in obese men. |
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Authors:
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Leonie K Heilbronn; Seng Khee Gan; Nigel Turner; Lesley V Campbell; Donald J Chisholm |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-23 |
Journal Detail:
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Title: The Journal of clinical endocrinology and metabolism Volume: 92 ISSN: 0021-972X ISO Abbreviation: J. Clin. Endocrinol. Metab. Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-05 Completed Date: 2007-09-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375362 Medline TA: J Clin Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: 1467-73 Citation Subset: AIM; IM |
Affiliation:
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Diabetes and Obesity Research Program, Garvan Institute for Medical Research, 384 Victoria Street, Darlinghurst, New South Wales 2010, Australia. l.heilbronn@garvan.org.au |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue
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anatomy & histology Adult Biological Markers Cyclooxygenase 1 / genetics Exercise Glucose Clamp Technique Humans Insulin / blood Insulin Resistance* Male Mitochondria, Muscle / genetics, metabolism, physiology* Muscle, Skeletal / cytology, pathology, physiopathology Obesity / physiopathology* Overweight / physiology* Oxygen Consumption Regression Analysis |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 11061-68-0/Insulin; EC 1.14.99.1/Cyclooxygenase 1; EC 1.14.99.1/PTGS1 protein, human |
| Comments/Corrections | |
Comment In:
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J Clin Endocrinol Metab. 2007 Apr;92(4):1229-31
[PMID:
17409341
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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