Document Detail

Markers of hepatitis C and B virus infections among blood donors in Ho Chi Minh City and Hanoi, Vietnam.
MedLine Citation:
PMID:  8556478     Owner:  NLM     Status:  MEDLINE    
Blood donors in two cities in Vietnam were tested for markers of hepatitis C virus (HCV) and hepatitis B virus infections. Antibody to HCV was detected by passive hemagglutination with antigens of the second generation in 101 (20.6%) of 491 donors in Ho Chi Minh City; it was detected less frequently (P < 0.001) in donors in hanoi (4 [0.8%] of 499). HCV RNA was tested for in donors with antibody by PCR with nested primers from the 5'-noncoding region and detected in 79 donors in Ho Chi Minh City and 4 donors in Hanoi. HCV RNA was genotyped by PCR with type-specific primers from the core gene. Of 83 HCV carriers from Vietnam, 24 (29%) were infected with HCV of genotype I/1a 19 (23%) were infected with II/1b, 4 (5%) were infected with III/2a, and 2 (2%) were infected with mixed genotypes (I/1a and II/1b); HCV genotypes in the remaining 34 (41%) donors, including all 4 donors in Hanoi, were not classifiable into I/1a, II/2a, IV/2b, or V/3a. Of the 10 isolates with unclassifiable genotypes, 2 showed substantial sequence divergence within the 5'-noncoding region from reported isolates with known genotypes (I/1a to 6a). An analysis of part of the core gene sequence indicated that six of the remaining isolates most likely represented new HCV genotypes. Hepatitis B surface antigen and the corresponding antibody, respectively, were detected in 15 (3.1%) and 234 (47.7%) donors in Ho Chi Minh City as well as 15 (3.0%) and 248 (49.7%) donors in Hanoi. These results indicate an extensive spread of HCV among Ho Chi Minh City donors and HCV of novel genotypes in vietnam.
P Song; D D Duc; B Hien; S Nakata; T Chosa; J Watanabe; F Tsuda; K Murata; H Okamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and diagnostic laboratory immunology     Volume:  1     ISSN:  1071-412X     ISO Abbreviation:  Clin. Diagn. Lab. Immunol.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1996-02-23     Completed Date:  1996-02-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9421292     Medline TA:  Clin Diagn Lab Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  413-8     Citation Subset:  IM; X    
Institute for Clinical Research in Tropical Medicine, Bach Mai Hospital, Hanoi, Vietnam.
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MeSH Terms
Age Factors
Base Sequence
Biological Markers / blood
Blood Donors*
HIV / immunology
Hepacivirus / genetics
Hepatitis B / blood*,  epidemiology,  immunology*
Hepatitis B Surface Antigens / immunology
Hepatitis C / blood*,  epidemiology,  immunology*
Hepatitis C Antigens / immunology
Human T-lymphotropic virus 1 / immunology
Middle Aged
Molecular Sequence Data
Seroepidemiologic Studies
Vietnam / epidemiology
Reg. No./Substance:
0/Biological Markers; 0/Hepatitis B Surface Antigens; 0/Hepatitis C Antigens

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