Document Detail

Markers of growth and development in primate primordial follicles are preserved after slow cryopreservation.
MedLine Citation:
PMID:  20074723     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the effect of slow cryopreservation on the morphology and function of primate primordial follicles within ovarian tissue slices.
DESIGN: Fresh monkey ovarian tissue was frozen by slow cryopreservation and thawed for analysis of morphologic and functional parameters.
SETTING: University-affiliated laboratory.
ANIMALS: Rhesus monkey ovarian tissue.
MAIN OUTCOME MEASURE(S): Histologic analysis, follicle counting, assessment of protein abundance and localization.
RESULT(S): After freezing and thawing, 89% of the primordial follicles maintained their laminar-based architecture, with sizes close to those of fresh fixed follicles. Molecular markers of early follicle health (activin subunits and the phosphorylated form of the signaling protein Smad2 [pSmad2]) were present in fresh and frozen-thawed primordial follicles. Stroma cells, but not follicles, had a higher level of TUNEL staining. Granulosa cells within the follicles of frozen-thawed ovarian tissue cultured for 48 hours had the capacity to proliferate and sustained expression of the activin subunits and nuclear pSmad2.
CONCLUSION(S): This study provides evidence that markers of early follicle growth and development are preserved after slow cryopreservation and thaw, with little effect on follicle morphology and function.
Shiying Jin; Lei Lei; Lonnie D Shea; Mary B Zelinski; Richard L Stouffer; Teresa K Woodruff
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-01-13
Journal Detail:
Title:  Fertility and sterility     Volume:  93     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-17     Completed Date:  2010-06-08     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2627-32     Citation Subset:  IM    
Copyright Information:
Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
Center for Reproductive Research, Northwestern University, Evanston, Illinois, USA.
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MeSH Terms
Activins / metabolism
Cell Proliferation
Cryopreservation / methods*,  veterinary
Macaca mulatta
Ovarian Follicle / cytology,  physiology*
Ovary / cytology*
Protein Subunits / metabolism
Smad2 Protein / metabolism
Tissue Culture Techniques
Grant Support
P01 HD021921-209001/HD/NICHD NIH HHS; P51 RR000163/RR/NCRR NIH HHS; P51 RR000163-496031/RR/NCRR NIH HHS; PL1 EB008542-03/EB/NIBIB NIH HHS; PL1EB008542/EB/NIBIB NIH HHS; RL1 HD058294-05/HD/NICHD NIH HHS; RL1 HD058295-01/HD/NICHD NIH HHS; RL1 HD058295-05/HD/NICHD NIH HHS; RL1-HD058295/HD/NICHD NIH HHS; RR00163/RR/NCRR NIH HHS; T90 1TL1CA133837/CA/NCI NIH HHS; TL1 CA133837-03/CA/NCI NIH HHS; U54 HD018185/HD/NICHD NIH HHS; U54 HD018185-16/HD/NICHD NIH HHS; U54-HD18185/HD/NICHD NIH HHS
Reg. No./Substance:
0/Protein Subunits; 0/Smad2 Protein; 104625-48-1/Activins

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