Document Detail

Marked increase in vascular endothelial growth factor concentrations during Escherichia coli endotoxin-induced acute inflammation in humans.
MedLine Citation:
PMID:  12925034     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Bacterial endotoxins can induce the synthesis and release of vascular endothelial growth factor (VEGF), which may alter vascular permeability and cause vascular leakage. MATERIALS AND METHODS: The effect of acute systemic inflammation on VEGF concentration was measured in healthy males after an intravenous bolus infusion of Escherichia coli endotoxin (lipopolysaccharide, LPS, 20 IU kg-1) in a double-blind, placebo-controlled parallel group study. LPS administration was followed by an infusion of lepirudin (bolus 0.1 mg kg-1, continuous infusion of 0.1 mg kg-1 h-1, n = 12) or saline (n = 12). RESULTS: Plasma VEGF increased from a mean of 15.1 pg mL-1 to 74.6 pg mL-1 5 h after LPS (P < 0.003). Body temperature, pulse rate, leukcytes, prothrombin fragment 1 + 2 (F1 + 2) and lactoferrin increased and platelets decreased after LPS (P < 0.05). The LPS-induced increase in VEGF was paralleled by the neutrophil cell degranulation marker lactoferrin but not by F1 + 2, and was not affected by lepirudin, which blunted F1 + 2 formation (P < 0.05). CONCLUSIONS: Inflammation-induced activation of leukcytes rather than platelets plays a role in the marked increase in VEGF, which cannot be abrogated by antithrombotic therapy.
F Mittermayer; J Pleiner; G Schaller; A Weltermann; S Kapiotis; B Jilma; M Wolzt
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  European journal of clinical investigation     Volume:  33     ISSN:  0014-2972     ISO Abbreviation:  Eur. J. Clin. Invest.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-19     Completed Date:  2003-11-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0245331     Medline TA:  Eur J Clin Invest     Country:  England    
Other Details:
Languages:  eng     Pagination:  758-61     Citation Subset:  IM    
Department of Clinical Pharmacology, University of Vienna, Vienna, Austria.
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MeSH Terms
Acute Disease
Anticoagulants / therapeutic use
Blood Coagulation / drug effects
Blood Platelets / drug effects,  physiology
Double-Blind Method
Endothelial Growth Factors / blood*
Escherichia coli*
Hirudins / analogs & derivatives*
Inflammation / blood*
Intercellular Signaling Peptides and Proteins / blood*
Leukocytes / drug effects,  physiology
Lymphokines / blood*
Recombinant Proteins / therapeutic use
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Reg. No./Substance:
0/Anticoagulants; 0/Endothelial Growth Factors; 0/Endotoxins; 0/Hirudins; 0/Intercellular Signaling Peptides and Proteins; 0/Lymphokines; 0/Recombinant Proteins; 0/Vascular Endothelial Growth Factor A; 0/Vascular Endothelial Growth Factors; 0/lepirudin

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