Document Detail


Marked increase in vascular endothelial growth factor concentrations during Escherichia coli endotoxin-induced acute inflammation in humans.
MedLine Citation:
PMID:  12925034     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Bacterial endotoxins can induce the synthesis and release of vascular endothelial growth factor (VEGF), which may alter vascular permeability and cause vascular leakage. MATERIALS AND METHODS: The effect of acute systemic inflammation on VEGF concentration was measured in healthy males after an intravenous bolus infusion of Escherichia coli endotoxin (lipopolysaccharide, LPS, 20 IU kg-1) in a double-blind, placebo-controlled parallel group study. LPS administration was followed by an infusion of lepirudin (bolus 0.1 mg kg-1, continuous infusion of 0.1 mg kg-1 h-1, n = 12) or saline (n = 12). RESULTS: Plasma VEGF increased from a mean of 15.1 pg mL-1 to 74.6 pg mL-1 5 h after LPS (P < 0.003). Body temperature, pulse rate, leukcytes, prothrombin fragment 1 + 2 (F1 + 2) and lactoferrin increased and platelets decreased after LPS (P < 0.05). The LPS-induced increase in VEGF was paralleled by the neutrophil cell degranulation marker lactoferrin but not by F1 + 2, and was not affected by lepirudin, which blunted F1 + 2 formation (P < 0.05). CONCLUSIONS: Inflammation-induced activation of leukcytes rather than platelets plays a role in the marked increase in VEGF, which cannot be abrogated by antithrombotic therapy.
Authors:
F Mittermayer; J Pleiner; G Schaller; A Weltermann; S Kapiotis; B Jilma; M Wolzt
Related Documents :
605804 - Rabbit polymorphonuclear leukocyte migration in vivo in response to lipopolysaccharides...
1548094 - Effect of brucella abortus lipopolysaccharide on oxidative metabolism and lysozyme rele...
11990084 - Immunotropic activity of vratizolin (itcl, denotivir).
24043374 - Higher body weight patients on clopidogrel maintenance therapy have lower active metabo...
7631404 - Platelet refractoriness and alloimmunization in pediatric oncology and bone marrow tran...
3590294 - The 24-hour shelf-life of cytapheresis platelet concentrates stored in polyvinyl chlori...
Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  European journal of clinical investigation     Volume:  33     ISSN:  0014-2972     ISO Abbreviation:  Eur. J. Clin. Invest.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-19     Completed Date:  2003-11-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0245331     Medline TA:  Eur J Clin Invest     Country:  England    
Other Details:
Languages:  eng     Pagination:  758-61     Citation Subset:  IM    
Affiliation:
Department of Clinical Pharmacology, University of Vienna, Vienna, Austria.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acute Disease
Adult
Anticoagulants / therapeutic use
Blood Coagulation / drug effects
Blood Platelets / drug effects,  physiology
Double-Blind Method
Endothelial Growth Factors / blood*
Endotoxins*
Escherichia coli*
Hirudins / analogs & derivatives*
Humans
Inflammation / blood*
Intercellular Signaling Peptides and Proteins / blood*
Leukocytes / drug effects,  physiology
Lymphokines / blood*
Male
Recombinant Proteins / therapeutic use
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Chemical
Reg. No./Substance:
0/Anticoagulants; 0/Endothelial Growth Factors; 0/Endotoxins; 0/Hirudins; 0/Intercellular Signaling Peptides and Proteins; 0/Lymphokines; 0/Recombinant Proteins; 0/Vascular Endothelial Growth Factor A; 0/Vascular Endothelial Growth Factors; 0/lepirudin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Are elevated homocysteine plasma levels related to peripheral arterial disease? Results from a cross...
Next Document:  Effects of bosentan on neointimal response following coronary angioplasty.