Document Detail


Marked inhibition of growth and invasive parameters of head and neck squamous carcinoma FaDu by a nutrient mixture.
MedLine Citation:
PMID:  19679626     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Head and neck squamous cell carcinomas (HNSCCs) are known for their aggressive growth and propensity to metastasize. The authors investigated the effect of a novel nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract on human HNSCC cell line FaDu in vivo and in vitro. Athymic male nude mice (n = 12) were inoculated with 3 x 10(6) FaDu cells subcutaneously and randomly divided into 2 groups: group A was fed a regular diet and group B a regular diet supplemented with 0.5% NM. Four weeks later, the mice were sacrificed and their tumors were excised, weighted, and processed for histology. In vitro, FaDu cells were cultured in Dulbecco's modified Eagle's medium and exposed to NM at 0 to 1000 microg/mL in triplicate. Cell proliferation was assessed by MTT assay, matrix metalloproteinase (MMP) secretion by gelatinase zymography, invasion through Matrigel, apoptosis by live-green caspases, and cell morphology by hematoxylin-eosin staining. NM inhibited the growth of tumors by 55% (P = .0002) and exhibited dose-dependent toxicity on FaDu cells in vitro, with 53% (P = .0003) at 1000 microg/mL NM. Zymography revealed MMP-2 and phorbol 12-myristate 13-acetate-induced MMP-9 secretion. NM inhibited secretion of both MMPs in a dose-dependent manner, with virtual total inhibition at 1000 microg/mL. NM significantly inhibited FaDu invasion through Matrigel with total block at 1000 microg/mL. NM induced dose-dependent apoptosis. In conclusion, NM has therapeutic potential in the treatment of HNSCC by significantly suppressing tumor growth and significantly inhibiting MMP secretion and invasion of HNSCC cells in vitro.
Authors:
M W Roomi; N W Roomi; T Kalinovsky; M Rath; A Niedzwiecki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Integrative cancer therapies     Volume:  8     ISSN:  1534-7354     ISO Abbreviation:  Integr Cancer Ther     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-08-14     Completed Date:  2009-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128834     Medline TA:  Integr Cancer Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  168-76     Citation Subset:  IM    
Affiliation:
Dr Rath Research Institute, Oncology Division, Santa Clara, California 95050, USA.
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Amino Acids / administration & dosage,  pharmacology,  therapeutic use
Animals
Apoptosis / drug effects
Ascorbic Acid / administration & dosage,  analogs & derivatives,  pharmacology,  therapeutic use
Camellia sinensis / chemistry
Carcinoma, Squamous Cell / drug therapy*,  metabolism,  pathology
Cell Line, Tumor
Cell Survival / drug effects
Complementary Therapies / methods*
Food*
Head and Neck Neoplasms / drug therapy*,  metabolism,  pathology
Humans
Male
Matrix Metalloproteinase 2 / metabolism
Matrix Metalloproteinase 9 / metabolism
Mice
Mice, Nude
Neoplasm Invasiveness
Plant Extracts / administration & dosage,  pharmacology,  therapeutic use
Tetradecanoylphorbol Acetate / pharmacology
Xenograft Model Antitumor Assays
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Plant Extracts; 16561-29-8/Tetradecanoylphorbol Acetate; 50-81-7/Ascorbic Acid; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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