| Marginal expression of CXCR4 on c-kit(+)Sca-1 (+)Lineage (-) hematopoietic stem/progenitor cells. | |
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MedLine Citation:
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PMID: 19937482 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stromal cell-derived factor 1 (SDF-1) and its receptor CXCR4 are the key regulatory molecules of hematopoietic stem cell (HSC) migration and engraftment to the bone marrow (BM) microenvironment. However, the significance of the ligand-receptor complex on HSC in steady-state BM is not clear. There is currently a lack of information as to how CXCR4 is expressed on HSCs. We herein demonstrate that c-kit(+)Sca-1(+)Lineage(-) (KSL) cells freshly isolated from BM expressed very low to undetectable levels of CXCR4. Two hours of incubation at 37 degrees C quickly up-modulated the receptor expression on KSL cells. Protein synthesis was not required for this early stage up-regulation, thus suggesting the emergence of intracellularly pooled receptors to the cell surface. However, protein synthesis was involved at the later stage of up-regulation. The up-regulated CXCR4 was functional, as evidenced by the fact that the incubated KSL cells more efficiently migrated to the SDF-1 gradient in vitro. Therefore, although KSL cells are able to express functional CXCR4, the receptors are only marginally expressed in the steady-state BM microenvironment. These observations therefore indicate the limited role of the SDF-1-CXCR4 axis on HSC functionality in a static BM environment. |
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Authors:
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Yutaka Sasaki; Yoshikazu Matsuoka; Makoto Hase; Takayuki Toyohara; Mari Murakami; Masaya Takahashi; Ryusuke Nakatsuka; Yasushi Uemura; Yoshiaki Sonoda |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-26 |
Journal Detail:
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Title: International journal of hematology Volume: 90 ISSN: 1865-3774 ISO Abbreviation: Int. J. Hematol. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2010-01-21 Completed Date: 2010-03-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9111627 Medline TA: Int J Hematol Country: United States |
Other Details:
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Languages: eng Pagination: 553-60 Citation Subset: IM |
Affiliation:
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Department of Stem Cell Biology and Regenerative Medicine, Graduate School of Medical Science, Kansai Medical University, Moriguchi, Osaka, 570-8506, Japan. sasakiy@takii.kmu.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Ly Bone Marrow Chemokine CXCL12 / physiology Hematopoietic Stem Cells Membrane Proteins Mice Mice, Inbred C57BL Proto-Oncogene Proteins c-kit Receptors, CXCR4 / analysis*, biosynthesis, physiology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Ly; 0/Chemokine CXCL12; 0/Cxcl12 protein, mouse; 0/Ly6a protein, mouse; 0/Membrane Proteins; 0/Receptors, CXCR4; EC 2.7.10.1/Proto-Oncogene Proteins c-kit |
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