| Marginal Biotin Deficiency Can Be Induced Experimentally in Humans Using a Cost-Effective Outpatient Design. | |
| | |
MedLine Citation:
|
PMID: 22157538 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
To date, marginal, asymptomatic biotin deficiency has been successfully induced experimentally by the use of labor-intensive inpatient designs requiring rigorous dietary control. We sought to determine if marginal biotin deficiency could be induced in humans in a less expensive outpatient design incorporating a self-selected, mixed general diet. We sought to examine the efficacy of three outpatient study designs: two based on oral avidin dosing and one based on a diet high in undenatured egg white for a period of 28 d. In study design 1, participants (n = 4; 3 women) received avidin in capsules with a biotin binding capacity of 7 times the estimated dietary biotin intake of a typical self-selected diet. In study design 2, participants (n = 2; 2 women) received double the amount of avidin capsules (14 times the estimated dietary biotin intake). In study design 3, participants (n = 5; 3 women) consumed egg-white beverages containing avidin with a biotin binding capacity of 7 times the estimated dietary biotin intake. Established indices of biotin status [lymphocyte propionyl-CoA carboxylase activity; urinary excretion of 3-hydroxyisovaleric acid, 3-hydroxyisovaleryl carnitine (3HIA-carnitine), and biotin; and plasma concentration of 3HIA-carnitine] indicated that study designs 1 and 2 were not effective in inducing marginal biotin deficiency, but study design 3 was as effective as previous inpatient study designs that induced deficiency by egg-white beverage. Marginal biotin deficiency can be induced experimentally by using a cost-effective outpatient design by avidin delivery in egg-white beverages. This design should be useful to the broader nutritional research community. |
| | |
Authors:
|
Shawna L Stratton; Cindy L Henrich; Nell I Matthews; Anna Bogusiewicz; Amanda M Dawson; Thomas D Horvath; Suzanne N Owen; Gunnar Boysen; Jeffery H Moran; Donald M Mock |
Related Documents
:
|
7549808 - Energy restriction and oxidative dna damage in humans. 6470818 - One cycle of reproduction consisting of pregnancy, lactation or no lactation, and recov... 15930808 - Effects of dietary restriction on physical performance in mice. 21781788 - Retinoids in eggs and embryos of birds fed fish from the great lakes. 12191848 - National differences in lipid response to postmenopausal hormone replacement therapy. 20471328 - Model of intestinal chylomicron over-production and ezetimibe treatment: impact on the ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-7 |
Journal Detail:
|
Title: The Journal of nutrition Volume: - ISSN: 1541-6100 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0404243 Medline TA: J Nutr Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Biochemistry and Molecular Biology, College of Medicine. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Prediction of Skeletal Muscle and Fat Mass in Patients with Advanced Cancer Using a Metabolomic Appr...
Next Document: The Historical Evolution of Thought Regarding Multiple Micronutrient Nutrition.