| Mapping of yeast Rpn4p transactivation domains. | |
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MedLine Citation:
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PMID: 18804109 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The 26S proteasome is a multi-subunit protease complex and plays an essential role in many basic cellular processes. The abundance of the 26S proteasome is controlled by a negative feedback circuit that involves the Rpn4p transcriptional activator. To date, the functional regions of Rpn4p are largely unknown. We mapped the Rpn4p transactivation domains by deletion analysis. The distal acidic domain has stronger transactivation potential than that of the proximal acidic domain. However, the N-terminal region, and not the acidic domains of Rpn4p, is crucial for Rpn4p function. Within the N-terminus, we mapped a novel transactivation domain, which may be regulated by some modification of lysines in a proteolysis-independent manner. |
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Authors:
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Dmitry S Karpov; Karpov S Dmitry; Vera V Tutyaeva; Vadim L Karpov |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-17 |
Journal Detail:
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Title: FEBS letters Volume: 582 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-11-03 Completed Date: 2008-12-10 Revised Date: 2008-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 3459-64 Citation Subset: IM |
Affiliation:
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Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Department of Chromatin Structure and Functions, Vavilov Strasse 32, Moscow 119991, Russia. aleom@yandex.ru |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acid Substitution Arginine / chemistry, genetics, metabolism Chromatin Immunoprecipitation DNA-Binding Proteins / chemistry*, genetics, metabolism Lysine / chemistry, genetics, metabolism Molecular Sequence Data Protein Structure, Tertiary / genetics Saccharomyces cerevisiae Proteins / chemistry*, genetics, metabolism Sequence Deletion Transcription Factors / chemistry*, genetics, metabolism Transcriptional Activation* |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/RPN4 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; 56-87-1/Lysine; 74-79-3/Arginine |
| Comments/Corrections | |
Erratum In:
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FEBS Lett. 2008 Nov 12;582(27):3845 Note: Dmitry, Karpov S [corrected to Karpov, Dmitry S] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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