Document Detail


Mapping of viral epitopes with conformationally specific monoclonal antibodies using biosensor technology.
MedLine Citation:
PMID:  1381370     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An automated biosensor system (BIAcore) designed for measuring molecular interactions in real time and without labelling any of the reactants was used for mapping the epitopes of tobacco mosaic virus protein using conformationally specific monoclonal antibodies (MAbs). Some of the MAbs used as capturing antibody on the sensor chip allowed a conformational change to occur in the viral protein. As a result, MAbs specific for the quaternary structure of polymerized viral protein were able to bind to monomeric viral subunits. Compared with classical solid-phase enzyme immunoassay, the biosensor technology possesses several advantages for epitope mapping of viral proteins.
Authors:
M C Dubs; D Altschuh; M H Van Regenmortel
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  597     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-10-06     Completed Date:  1992-10-06     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  391-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal*
Antigens, Viral / analysis,  immunology*
Biosensing Techniques*
Enzyme-Linked Immunosorbent Assay
Epitopes / analysis*
Genes, Viral
Protein Conformation
Tobacco Mosaic Virus / genetics,  immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Viral; 0/Epitopes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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