| Mapping of viral epitopes with conformationally specific monoclonal antibodies using biosensor technology. | |
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MedLine Citation:
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PMID: 1381370 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An automated biosensor system (BIAcore) designed for measuring molecular interactions in real time and without labelling any of the reactants was used for mapping the epitopes of tobacco mosaic virus protein using conformationally specific monoclonal antibodies (MAbs). Some of the MAbs used as capturing antibody on the sensor chip allowed a conformational change to occur in the viral protein. As a result, MAbs specific for the quaternary structure of polymerized viral protein were able to bind to monomeric viral subunits. Compared with classical solid-phase enzyme immunoassay, the biosensor technology possesses several advantages for epitope mapping of viral proteins. |
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Authors:
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M C Dubs; D Altschuh; M H Van Regenmortel |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography Volume: 597 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1992 Apr |
Date Detail:
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Created Date: 1992-10-06 Completed Date: 1992-10-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 391-6 Citation Subset: IM |
Affiliation:
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Institut de Biologie Moléculaire et Cellulaire, Strasbourgh, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal* Antigens, Viral / analysis, immunology* Biosensing Techniques* Enzyme-Linked Immunosorbent Assay Epitopes / analysis* Genes, Viral Protein Conformation Tobacco Mosaic Virus / genetics, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, Viral; 0/Epitopes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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