Document Detail

Mapping of two isoleucine tRNA isoacceptor genes in bacteriophage T5 DNA.
MedLine Citation:
PMID:  978797     Owner:  NLM     Status:  MEDLINE    
T5 bacteriophage codes for the synthesis of more than 14 different tRNA species, which map in four separate clusters in the C segment of the T5 chromosome. In this study, two tRNAile isoacceptor species have been identified by reverse-phase chromatography and shown to be transcribed from two different tRNA loci along the T5 chromosome. The map positions of the tRNA isoacceptors were aided by the use of several T5 deletion mutants in which the position and size of the deleted DNA segments had been previously determined by heteroduplex mapping. Hybridization analysis suggests the presence of some sequence homology between the two tRNAile species.
C Hunt; L T Hwang; S B Weiss
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  20     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1976 Oct 
Date Detail:
Created Date:  1976-12-23     Completed Date:  1976-12-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  63-9     Citation Subset:  IM    
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MeSH Terms
Chromosome Mapping
Coliphages / metabolism*
DNA, Viral*
Nucleic Acid Conformation
RNA, Transfer / biosynthesis*
RNA, Viral / biosynthesis*
Transcription, Genetic
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Viral; 9014-25-9/RNA, Transfer

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