Document Detail

Mapping of the thymidine kinase genes of type 1 and type 2 herpes simplex viruses using intertypic recombinants.
MedLine Citation:
PMID:  6255066     Owner:  NLM     Status:  MEDLINE    
The thymidine kinase induced in lytic infection by each of 36 intertypic recombinants of herpes simplex virus (HSV) type 1 and type 2 was identified as type 1 or type 2 by studies on the thermolability of enzyme activity, neutralization with type 1 or type 2 antiserum and agar gel immunodiffusion with type 1 or type 2 thymidine kinase antiserum. Fourteen recombinants induced no thymidine kinase, 12 induced type 1 thymidine kinase and 10 induced type 2 thymidine kinase. Correlation of these results with restriction endonuclease analysis of the DNA of the recombinants with five restriction endonucleases (XbaI, EcoRI, HpaI, HsuI, and Bg/II) allowed mapping of the type 1 thymidine kinase gene at 0.300 to 0.309 map unit and the type 2 thymidine kinase gene at 0.295 to 0.315 map unit.
I W Halliburton; L S Morse; B Roizman; K E Quinn
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of general virology     Volume:  49     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  1980 Aug 
Date Detail:
Created Date:  1981-02-26     Completed Date:  1981-02-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  235-53     Citation Subset:  IM    
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MeSH Terms
Chromosome Mapping
DNA Restriction Enzymes
DNA, Viral
Recombination, Genetic
Simplexvirus / genetics*
Thymidine Kinase / genetics*,  immunology
Grant Support
Reg. No./Substance:
0/DNA, Viral; EC Kinase; EC 3.1.21.-/DNA Restriction Enzymes

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