| Mapping of the thymidine kinase genes of type 1 and type 2 herpes simplex viruses using intertypic recombinants. | |
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MedLine Citation:
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PMID: 6255066 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The thymidine kinase induced in lytic infection by each of 36 intertypic recombinants of herpes simplex virus (HSV) type 1 and type 2 was identified as type 1 or type 2 by studies on the thermolability of enzyme activity, neutralization with type 1 or type 2 antiserum and agar gel immunodiffusion with type 1 or type 2 thymidine kinase antiserum. Fourteen recombinants induced no thymidine kinase, 12 induced type 1 thymidine kinase and 10 induced type 2 thymidine kinase. Correlation of these results with restriction endonuclease analysis of the DNA of the recombinants with five restriction endonucleases (XbaI, EcoRI, HpaI, HsuI, and Bg/II) allowed mapping of the type 1 thymidine kinase gene at 0.300 to 0.309 map unit and the type 2 thymidine kinase gene at 0.295 to 0.315 map unit. |
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Authors:
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I W Halliburton; L S Morse; B Roizman; K E Quinn |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of general virology Volume: 49 ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 1980 Aug |
Date Detail:
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Created Date: 1981-02-26 Completed Date: 1981-02-26 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 235-53 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromosome Mapping DNA Restriction Enzymes DNA, Viral Immunodiffusion Recombination, Genetic Simplexvirus / genetics* Temperature Thymidine Kinase / genetics*, immunology |
| Grant Support | |
ID/Acronym/Agency:
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CA-08494/CA/NCI NIH HHS; CA-19264/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; EC 2.7.1.21/Thymidine Kinase; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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