| Mapping system for coregistration of cardiac mri and ex vivo tissue sampling. | |
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MedLine Citation:
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PMID: 21932357 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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PURPOSE: To design a method suitable for obtaining tissue samples from regions of different function as ascertained by magnetic resonance imaging (MRI). MATERIALS AND METHODS: In vivo MRI was used to create azimuthal projections of the heart from dilated cardiomyopathy transplant patients with the cardiac valves in the center and four concentric rings representing the septum and free wall. Tagged MRI could identify regions of different contractile strength that were then transferred onto the map projection. The resulting tissue sampling map was used to guide dissection of tissue samples from the explanted heart for analysis by electron microscopy (EM) as well as provide samples for subsequent mRNA analysis. Accuracy of the sampling was determined in a sheep heart using 17 fiduciary markers glued to the epicardial surface. RESULTS: Tagged MRI identified areas of "normal" (%S(c) -11), "poor" (%S(c) -4) and "failed" contraction (%S(c) +3). The mapping method we developed enabled straightforward sampling of these regions after surgical excision. EM showed good tissue preservation while the test of accuracy using the fiduciary markers showed a sampling accuracy of 0.3 ± 3.7 mm. This was similar to the resolution of tagged MRI images themselves. CONCLUSION: The methods we have developed can accurately guide tissue sampling for ex vivo tissue analysis. J. Magn. Reson. Imaging 2011. © 2011 Wiley-Liss, Inc. |
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Authors:
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Alistair A Young; David J Crossman; Peter N Ruygrok; Mark B Cannell |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-19 |
Journal Detail:
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Title: Journal of magnetic resonance imaging : JMRI Volume: - ISSN: 1522-2586 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9105850 Medline TA: J Magn Reson Imaging Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
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Department of Anatomy with Radiology, University of Auckland, Auckland, New Zealand. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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