| Mapping of surrogate markers of cellular components and structures using laser desorption/ionization mass spectrometry. | |
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MedLine Citation:
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PMID: 10679989 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Laser desorption/ionization mass spectrometry (LDI-MS) has been used to assess the potential of using surrogate markers, bound to cellular structures containing nucleic acids, to image or map the position of these structures within biological samples. In this study, organic dyes were used as markers because of their established use in the histochemical marking of nucleic acids, and also because they are amenable to LDI-MS. Eight cationic dyes were tested and all could be desorbed from nucleic acid samples without additional matrix after specifically binding to these molecules. Methylene Blue was the best of these based on its sensitivity to detection by LDI-MS and the fact that it can be washed from the tissue in areas where it was not specifically bound to provide low-intensity background signals. Experiments are reported which characterize the M(+) ion signal obtained from Methylene Blue with regard to sensitivity, reproducibility and possible use for quantitation. This dye was used to map (with a lateral resolution of 25 microm) several nucleic acid-containing samples spotted on prepared surfaces, and to image the location of nucleic acids in two model tissues, retinal vertical sections and thyroid whole mount sections. |
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Authors:
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J M Koomen; M Stoeckli; R M Caprioli |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of mass spectrometry : JMS Volume: 35 ISSN: 1076-5174 ISO Abbreviation: J Mass Spectrom Publication Date: 2000 Feb |
Date Detail:
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Created Date: 2000-03-28 Completed Date: 2000-03-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9504818 Medline TA: J Mass Spectrom Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 258-64 Citation Subset: IM |
Copyright Information:
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Copyright 2000 John Wiley & Sons, Ltd. |
Affiliation:
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Laboratory for Biological Mass Spectrometry, Chemistry Department, Texas A&M University, College Station, Texas 77842, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers Cells / chemistry* Coloring Agents / chemistry DNA / chemistry Methylene Blue / chemistry Microscopy, Fluorescence Nucleic Acids / analysis Rabbits Rats Retina / chemistry, cytology Saccharomyces cerevisiae / chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thyroid Gland / chemistry, cytology |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Coloring Agents; 0/Nucleic Acids; 61-73-4/Methylene Blue; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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