| Mapping the speciation of iron in Pseudomonas aeruginosa biofilms using scanning transmission X-ray microscopy. | |
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MedLine Citation:
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PMID: 19192795 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An important feature of microbial communities is the spatial heterogeneity of extracellular chemistry. Scanning transmission X-ray microscopy (STXM) was used to map the spatial distribution of iron speciesthroughout Pseudomonas aeruginosa biofilms to assess the influence of chemical heterogeneity on biomineralization. P. aeruginosa biofilms were treated with Fe(III)-amended media. Speciation and quantitative mapping using STXM image sequences in the Fe 2p(3/2) (L3) absorption edge region revealed both Fe(II) and Fe(III) in localized microenvironments. Fe(III) was mainly associated with cell surfaces, while small amounts of Fe(II) was found in the extracellular space. Biofilms were also characterized using C 1s edge STXM image sequences. Anaerobic growth assays and confocal microscopy revealed the inability of P. aeruginosa to directly reduce Fe(III), implicating indirect iron reduction mechanisms in the formation of fine-grained, multivalent minerals. These studies suggest that geochemical microenvironments found throughout microbial communities are even more complex than previously believed. |
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Authors:
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Ryan C Hunter; Adam P Hitchcock; James J Dynes; Martin Obst; Terry J Beveridge |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Environmental science & technology Volume: 42 ISSN: 0013-936X ISO Abbreviation: Environ. Sci. Technol. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2009-02-05 Completed Date: 2009-03-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0213155 Medline TA: Environ Sci Technol Country: United States |
Other Details:
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Languages: eng Pagination: 8766-72 Citation Subset: IM |
Affiliation:
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Molecular and Cellular Biology, University of Guelph, Guelph, Ontario. rchunter@mit.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biodegradation, Environmental
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drug effects Biofilms / drug effects, growth & development* Cryoultramicrotomy Freeze Drying Green Fluorescent Proteins / metabolism Iron / metabolism*, pharmacology Microscopy / methods* Microscopy, Confocal Microscopy, Electron, Transmission Pseudomonas aeruginosa / cytology, physiology*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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147336-22-9/Green Fluorescent Proteins; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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