Document Detail


Mapping the speciation of iron in Pseudomonas aeruginosa biofilms using scanning transmission X-ray microscopy.
MedLine Citation:
PMID:  19192795     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An important feature of microbial communities is the spatial heterogeneity of extracellular chemistry. Scanning transmission X-ray microscopy (STXM) was used to map the spatial distribution of iron speciesthroughout Pseudomonas aeruginosa biofilms to assess the influence of chemical heterogeneity on biomineralization. P. aeruginosa biofilms were treated with Fe(III)-amended media. Speciation and quantitative mapping using STXM image sequences in the Fe 2p(3/2) (L3) absorption edge region revealed both Fe(II) and Fe(III) in localized microenvironments. Fe(III) was mainly associated with cell surfaces, while small amounts of Fe(II) was found in the extracellular space. Biofilms were also characterized using C 1s edge STXM image sequences. Anaerobic growth assays and confocal microscopy revealed the inability of P. aeruginosa to directly reduce Fe(III), implicating indirect iron reduction mechanisms in the formation of fine-grained, multivalent minerals. These studies suggest that geochemical microenvironments found throughout microbial communities are even more complex than previously believed.
Authors:
Ryan C Hunter; Adam P Hitchcock; James J Dynes; Martin Obst; Terry J Beveridge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Environmental science & technology     Volume:  42     ISSN:  0013-936X     ISO Abbreviation:  Environ. Sci. Technol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-02-05     Completed Date:  2009-03-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0213155     Medline TA:  Environ Sci Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8766-72     Citation Subset:  IM    
Affiliation:
Molecular and Cellular Biology, University of Guelph, Guelph, Ontario. rchunter@mit.edu
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MeSH Terms
Descriptor/Qualifier:
Biodegradation, Environmental / drug effects
Biofilms / drug effects,  growth & development*
Cryoultramicrotomy
Freeze Drying
Green Fluorescent Proteins / metabolism
Iron / metabolism*,  pharmacology
Microscopy / methods*
Microscopy, Confocal
Microscopy, Electron, Transmission
Pseudomonas aeruginosa / cytology,  physiology*,  ultrastructure
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins; 7439-89-6/Iron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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