Document Detail


Mapping of independent V3 envelope determinants of human immunodeficiency virus type 1 macrophage tropism and syncytium formation in lymphocytes.
MedLine Citation:
PMID:  8971043     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The V3 region of the human immunodeficiency virus type 1 (HIV-1) envelope protein is known to have a major influence on macrophage tropism as well as the ability to cause syncytium formation or fusion in CD4-positive lymphocyte cultures. Using infectious molecular HIV-1 clones, a series of mutant clones was created which allowed detailed mapping of V3 amino acid positions involved in these properties. In these experiments the non-syncytium-inducing phenotype in T cells did not always correlate with macrophage tropism. Macrophage tropism appeared to depend on the presence of certain combinations of amino acids at five specific positions within and just outside of the V3 loop itself, whereas syncytium formation in lymphocytes was influenced by substitution of particular residues at two to four positions within V3. In most cases, different V3 amino acid positions were found to independently influence macrophage tropism and syncytium formation in T cells and position 13 was the only V3 location which appeared to simultaneously influence both macrophage tropism and syncytium formation in lymphocytes.
Authors:
B Chesebro; K Wehrly; J Nishio; S Perryman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  70     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1996 Dec 
Date Detail:
Created Date:  1997-01-23     Completed Date:  1997-01-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9055-9     Citation Subset:  IM; X    
Affiliation:
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA. bchesebro@atlas.niaid.nih.gov
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
CD4-Positive T-Lymphocytes / immunology*
Epitopes / chemistry,  immunology*
Giant Cells
HIV Envelope Protein gp120 / chemistry*,  genetics,  immunology*
HIV Infections / immunology*,  virology
HIV-1 / genetics,  immunology*
Hela Cells
Humans
Macrophages / immunology*
Molecular Sequence Data
Peptide Fragments / chemistry*,  genetics,  immunology*
Structure-Activity Relationship
Chemical
Reg. No./Substance:
0/Epitopes; 0/HIV Envelope Protein gp120; 0/HIV envelope protein gp120 (305-321); 0/Peptide Fragments
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