| Mapping and functional analysis of regulatory sequences in the mouse lambda5-VpreB1 domain. | |
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MedLine Citation:
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PMID: 15950724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The lambda5 and VpreB genes encode the components of the surrogate light-chain which forms part of the pre-B cell receptor and plays a key role in B cell development. In the mouse, the lambda5 and VpreB1 genes are closely linked and are co-regulated by a multi-component locus control region. To identify the sequences that regulate lambda5 and VpreB1 expression during B cell development, we have comprehensively mapped the DNaseI hypersensitive sites (HS) in the lambda5-VpreB1 functional domain. The active domain contains 12 HS that are distributed at high density across the 18.3 kb region that forms the lambda5 and VpreB1 functional unit. Analysis of a reporter gene driven by the VpreB1 promoter in transgenic mice identified a novel enhancer associated with two HS located upstream of lambda5. The lambda5-VpreB1 locus was also found to be closely linked to the ubiquitously expressed Topoisomerase-3beta (Topo3beta) gene. The VpreB1 and Topo3beta genes have entirely different expression patterns despite the fact that the two promoters are separated by a distance of only 1.5 kb. |
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Authors:
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Sophia Minaee; Deborah Farmer; Andrew Georgiou; Pierangela Sabbattini; Zoe Webster; Cheok-Man Chow; Niall Dillon |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-02-26 |
Journal Detail:
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Title: Molecular immunology Volume: 42 ISSN: 0161-5890 ISO Abbreviation: Mol. Immunol. Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-06-13 Completed Date: 2005-08-12 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7905289 Medline TA: Mol Immunol Country: England |
Other Details:
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Languages: eng Pagination: 1283-92 Citation Subset: IM |
Affiliation:
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Gene regulation and Chromatin Group, MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College, Hammersmith Campus, Du Cane Road, London W12 ONN, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals B-Lymphocytes / cytology, immunology Base Sequence Cell Differentiation Cell Line Chromosome Mapping DNA, Complementary / genetics Deoxyribonuclease I Enhancer Elements, Genetic Genes, Regulator Immunoglobulin Light Chains / genetics* Immunoglobulin Light Chains, Surrogate Immunoglobulin Variable Region / genetics* Membrane Glycoproteins / genetics* Mice Mice, Transgenic Plasmids / genetics Pre-B Cell Receptors Promoter Regions, Genetic Receptors, Antigen, B-Cell |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Immunoglobulin Light Chains; 0/Immunoglobulin Light Chains, Surrogate; 0/Immunoglobulin Variable Region; 0/Membrane Glycoproteins; 0/Pre-B Cell Receptors; 0/Receptors, Antigen, B-Cell; EC 3.1.21.1/Deoxyribonuclease I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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