Document Detail


Mapping and comparison of the B-cell epitopes recognized on the Plasmodium vivax circumsporozoite protein by immune Colombians and immunized Aotus monkeys.
MedLine Citation:
PMID:  9797827     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plasma samples of individuals from two malaria-endemic villages on the Colombian Pacific coast and synthetic peptides representing different fragments of the central and flanking regions of the Plasmodium vivax circumsporozoite protein (CSP) were used to perform a fine mapping of the B-cell epitopes on the whole CSP. In addition, the immunogenicity of long polypeptides corresponding to the amino (N) and carboxyl (C) regions was evaluated in Aotus monkeys. The epitopes recognized after natural infection of humans and after immunization of Aotus with these synthetic peptides were compared. Human samples more frequently contained specific antibodies to the central region. The type-I repeat region of the CSP was predominantly recognized by the human sera (by 68% of those from the village of Zacarías and 75% of those from Bajo Calima), a significantly smaller population reacting with the type-II repeat (20% and 11%, respectively). Most of the sera reacting with the type-I repeat recognized the minimal epitope AGDR. Although the N- and C-terminal polypeptides were both highly immunogenic in Aotus and induced long-lasting antibodies, titres of antibodies to the C-terminal polypeptide were higher than those of antibodies to the N-terminal. Competitive inhibition assays performed using human and monkey plasma allowed the identification of dominant B-cell epitopes on sequence 71-90 (p8) from the amino region and sequence 332-361 (p24/p25) from the carboxyl region. The high prevalence of naturally induced antibodies to the three epitopes, the possible functional role of the corresponding sequences, and the high immunogenicity of these epitopes in Aotus could be of great importance in the development of a malaria vaccine based on P. vivax CSP.
Authors:
M Arévalo-Herrera; M A Roggero; J M Gonzalez; J Vergara; G Corradin; J A López; S Herrera
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of tropical medicine and parasitology     Volume:  92     ISSN:  0003-4983     ISO Abbreviation:  Ann Trop Med Parasitol     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-11-13     Completed Date:  1998-11-13     Revised Date:  2009-05-18    
Medline Journal Info:
Nlm Unique ID:  2985178R     Medline TA:  Ann Trop Med Parasitol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  539-51     Citation Subset:  IM    
Affiliation:
Instituto de Inmunología, Universidad del Valle, Cali, Colombia. marami@sinsa.univalle.edu.co
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Animals
Antibodies, Protozoan / analysis
Aotidae
B-Lymphocytes / immunology*
Colombia
Enzyme-Linked Immunosorbent Assay
Epitope Mapping
Epitopes / analysis*
Female
Humans
Lymphocyte Activation
Malaria, Vivax / immunology
Male
Middle Aged
Plasmodium vivax / immunology*
Protozoan Proteins / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Protozoan; 0/Epitopes; 0/Protozoan Proteins; 0/circumsporozoite protein, Protozoan

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