Document Detail


Manufacturing and banking of mesenchymal stem cells.
MedLine Citation:
PMID:  23339745     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Mesenchymal stem cells (MSC) and MSC-like cells hold great promise and offer many advantages for developing effective cellular therapeutics. Current trends indicate that the clinical application of MSC will continue to increase markedly. For clinical applications, large numbers of MSC are usually required, ideally in an off-the-shelf format, thus requiring extensive MSC expansion ex vivo and subsequent cryopreservation and banking.
AREAS COVERED: To exploit the full potential of MSC for cell-based therapies requires overcoming significant cell-manufacturing, banking and regulatory challenges. The current review will focus on the identification of optimal cell source for MSC, the techniques for production scale-up, cryopreservation and banking and the regulatory challenges involved.
EXPERT OPINION: There has been considerable success manufacturing and cryopreserving MSC at laboratory scale. Surprisingly little attention, however, has been given to translate these technologies to an industrial scale. The development of cost-effective advanced technologies for producing and cryopreserving commercial-scale MSC is important for successful clinical cell therapy.
Authors:
Sreedhar Thirumala; W Scott Goebel; Erik J Woods
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2013-01-23
Journal Detail:
Title:  Expert opinion on biological therapy     Volume:  13     ISSN:  1744-7682     ISO Abbreviation:  Expert Opin Biol Ther     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-08     Completed Date:  2013-09-25     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  101125414     Medline TA:  Expert Opin Biol Ther     Country:  England    
Other Details:
Languages:  eng     Pagination:  673-91     Citation Subset:  IM    
Affiliation:
Cook General Biotechnology LLC, Indianapolis, IN 46202, USA.
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MeSH Terms
Descriptor/Qualifier:
Bioreactors
Cell Culture Techniques
Cell Differentiation / physiology
Cells, Cultured
Cryopreservation
Drug Industry
Guidelines as Topic
Humans
Legislation, Medical
Mesenchymal Stromal Cells* / physiology
Tissue Banks / organization & administration*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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