Document Detail

Mannose-exposing myeloid leukemia cells detected by the sCAR-PPA fusion protein.
MedLine Citation:
PMID:  19377843     Owner:  NLM     Status:  MEDLINE    
Altered glycosylation may be a hallmark of malignant transformation and cancer progression. In the work described, a specific mannose-binding lectin, Pinellia pedatisecta agglutinin (PPA), was genetically fused with the extracellular domain of coxsackie-adenovirus receptor (CAR) to generate the soluble CAR (sCAR)-PPA fusion protein. The adenoviral transduction of acute myeloid leukemia (AML) cell lines Kasumi-1 and HL-60 was increased by sCAR-PPA, indicating that a fraction of AML cells exposing mannose residues was detected by PPA. However, sCAR-PPA did not increase the adenoviral infection of KG-1 cells, suggesting the mannose exposure of AML cells may be cell type specific. Furthermore, the infectious efficiency of Ad-EGFP in chronic myeloid leukemia cell line K562 was significantly increased by sCAR-PPA as well. We, herein, report that PPA recognized a fraction of myeloid leukemia cells showing mannose-exposing phenotype. The sCAR-PPA fusion protein combined with the adenoviral vector system may provide a useful tool for investigating myeloid leukemia cells exposing mannose residues and further elucidating the role of these cells in the leukemia development.
Gong Chu Li; Na Li; Yan Hong Zhang; Xin Li; Yi Gang Wang; Xin Yuan Liu; Wen Bin Qian; Xiao Chuan Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-18
Journal Detail:
Title:  International journal of hematology     Volume:  89     ISSN:  1865-3774     ISO Abbreviation:  Int. J. Hematol.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-02     Completed Date:  2009-09-09     Revised Date:  2011-07-01    
Medline Journal Info:
Nlm Unique ID:  9111627     Medline TA:  Int J Hematol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  611-7     Citation Subset:  IM    
Xin Yuan Institute of Medicine and Biotechnology, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, People's Republic of China.
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MeSH Terms
Agglutinins / diagnostic use*,  genetics
Cell Line, Tumor
Leukemia, Myeloid / diagnosis,  pathology*
Mannose / metabolism*
Mannose-Binding Lectin / diagnostic use*
Pinellia / genetics
Receptors, Virus / genetics
Recombinant Fusion Proteins / diagnostic use
Reg. No./Substance:
0/Agglutinins; 0/CXADR-like membrane protein; 0/Mannose-Binding Lectin; 0/Receptors, Virus; 0/Recombinant Fusion Proteins; 31103-86-3/Mannose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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